Mouse Chemokine (C motif) ligand (XCL1) ELISA Kit | XCL1 elisa kit
Mouse Chemokine (C motif) ligand (XCL1) Mini Samples ELISA Kit
Gene Names
Xcl1; LTN; ATAC; Lptn; SCM-1; Scyc1; SCM-1a; AI661682
Reactivity
Mouse
Applications
ELISA
Synonyms
Chemokine (C motif) ligand (XCL1); Mouse Chemokine (C motif) ligand (XCL1) Mini Samples ELISA Kit; Chemokine (C motif) ligand; Lptn; SCYC1; LTN; ATAC; SCM1-A; SCM1a; LPTN; Lymphotactin; Lymphotaxin; Chemokine C-Motif Ligand 1; Small Inducible Cytokine Subfamily C; Member 1; XC chemokine ligand 1; XCL1 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Chemokine (C motif) ligand (XCL1).
No significant cross-reactivity or interference between Mini Samples Chemokine (C motif) ligand (XCL1) and analogues was observed.
No significant cross-reactivity or interference between Mini Samples Chemokine (C motif) ligand (XCL1) and analogues was observed.
Sequence Length
114
Applicable Applications for XCL1 elisa kit
ELISA (EIA)
Assay Type
Double-antibody Sandwich
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Chemokine (C motif) ligand (XCL1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Chemokine (C motif) ligand (XCL1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Chemokine (C motif) ligand (XCL1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Chemokine (C motif) ligand (XCL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Chemokine (C motif) ligand (XCL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Chemokine (C motif) ligand (XCL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Chemokine (C motif) ligand (XCL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Lymphotactin
NCBI Official Synonym Full Names
chemokine (C motif) ligand 1
NCBI Official Symbol
Xcl1
NCBI Official Synonym Symbols
LTN; ATAC; Lptn; SCM-1; Scyc1; SCM-1a; AI661682
NCBI Protein Information
lymphotactin
UniProt Protein Name
Lymphotactin
Protein Family
UniProt Gene Name
Xcl1
UniProt Synonym Gene Names
Lptn; Ltn; Scyc1
UniProt Entry Name
XCL1_MOUSE
Uniprot Description
XCL1: Chemotactic activity for lymphocytes but not for monocytes or neutrophils. Belongs to the intercrine gamma family.
Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide
Cellular Component: extracellular space; cell; extracellular region; intracellular; cytosol
Molecular Function: protein homodimerization activity; chemokine activity; chemokine receptor binding; cytokine activity
Biological Process: positive regulation of T cell mediated cytotoxicity; response to virus; negative regulation of transcription factor activity; positive regulation of T cell cytokine production; positive regulation of leukocyte chemotaxis; positive regulation of immune response to tumor cell; negative regulation of T cell cytokine production; chemotaxis; positive regulation of interleukin-10 production; negative regulation of interleukin-2 production; cell-cell signaling; release of sequestered calcium ion into cytosol; negative regulation of interferon-gamma production; regulation of inflammatory response; positive regulation of innate immune response; immune response; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of transcription, DNA-dependent
Research Articles on XCL1
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Product Notes
The Mouse XCL1 xcl1 (Catalog #AAA2708329) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2708329 ELISA Kit recognizes Mouse XCL1. AAA Biotech's Chemokine (C motif) ligand (XCL1) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the XCL1 xcl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Chemokine (C motif) ligand (XCL1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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