Human Ceruloplasmin (CP) CLIA Kit | CP clia kit

Human Ceruloplasmin (CP) CLIA Kit

Cat. Num. AAA2000726

• Gene Names: CP; CP-2
• Reactivity: Human
• Synonyms: Ceruloplasmin (CP); Human Ceruloplasmin (CP) CLIA Kit; CP2; CER; Caeruloplasmin; Ferroxidase; Oxygen Oxidoreductase; CP clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ceruloplasmin (CP).; No significant cross-reactivity or interference between Ceruloplasmin (CP) and analogues was observed.
• Sequence Length: 1065

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other Biological Fluids
• Detection Range: 1.37-1,000pg/mL
• Sensitivity: < 0.58pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ceruloplasmin (CP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ceruloplasmin (CP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CP clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Ceruloplasmin (CP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ceruloplasmin (CP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ceruloplasmin (CP) level in the sample or standard.;

Product Categories/Family for CP clia kit

Metabolic pathway; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 4557485
• NCBI GeneID: 1356
• NCBI Accession #: NP_000087.1
• NCBI GenBank Nucleotide #: NM_000096.3
• UniProt Accession #: P00450; Q14063; Q2PP18; Q9UKS4
• Molecular Weight: 122,205 Da
• NCBI Official Full Name: ceruloplasmin
• NCBI Official Synonym Full Names: ceruloplasmin (ferroxidase)
• NCBI Official Symbol: CP
• NCBI Official Synonym Symbols: CP-2
• NCBI Protein Information: ceruloplasmin
• UniProt Protein Name: Ceruloplasmin
• Protein Family: Ceruloplasmin
• UniProt Gene Name: CP
• UniProt Entry Name: CERU_HUMAN

NCBI Description

The protein encoded by this gene is a metalloprotein that binds most of the copper in plasma and is involved in the peroxidation of Fe(II)transferrin to Fe(III) transferrin. Mutations in this gene cause aceruloplasminemia, which results in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

CP: Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense. Defects in CP are the cause of aceruloplasminemia (ACERULOP). It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances. Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2. Belongs to the multicopper oxidase family.

Protein type: Oxidoreductase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.16.3.1; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q23-q25

Cellular Component: extracellular space; lysosomal membrane; extracellular region

Molecular Function: ferroxidase activity; copper ion binding; chaperone binding

Biological Process: cellular iron ion homeostasis; copper ion transport; transmembrane transport

Disease: Aceruloplasminemia