Typical Testing Data/Standard Curve (for reference only)
Mouse Ceramide Kinase ELISA Kit | CERK elisa kit
Mouse Ceramide Kinase ELISA Kit
Gene Names
CERK; LK4; hCERK; dA59H18.2; dA59H18.3
Reactivity
Mouse
Synonyms
Ceramide Kinase; Mouse Ceramide Kinase ELISA Kit; CERK elisa kit
Reactivity
Mouse
Sequence Length
537
Samples
Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
7ng/L-1500ng/L
Sensitivity
4.03ng/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CERK elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse C-C Motif Chemokine 27 (also known as CCL27) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse CCL27 antibody. CCL27 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CCL27 Antibody is added and binds to CCL27 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CCL27 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse CCL27. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse CCL27 antibody. CCL27 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CCL27 Antibody is added and binds to CCL27 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CCL27 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse CCL27. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ceramide kinase
NCBI Official Synonym Full Names
ceramide kinase
NCBI Official Symbol
CERK
NCBI Official Synonym Symbols
LK4; hCERK; dA59H18.2; dA59H18.3
NCBI Protein Information
ceramide kinase
UniProt Protein Name
Ceramide kinase
Protein Family
UniProt Gene Name
CERK
UniProt Synonym Gene Names
KIAA1646; hCERK; LK4
NCBI Description
CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]).[supplied by OMIM, Mar 2008]
Uniprot Description
Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides.
Research Articles on CERK
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Product Notes
The Mouse CERK cerk (Catalog #AAA1603505) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1603505 ELISA Kit recognizes Mouse CERK. It is sometimes possible for the material contained within the vial of "Ceramide Kinase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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