Human Ceramide kinase (CERK) ELISA Kit | CERK elisa kit

Human Ceramide kinase (CERK) ELISA Kit

Cat. Num. AAA7210885

• Gene Names: CERK; LK4; hCERK; dA59H18.2; dA59H18.3
• Reactivity: Human
• Synonyms: Ceramide kinase (CERK); Human Ceramide kinase (CERK) ELISA Kit; CERK elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This CERK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CERK. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CERK elisa kit

Principle of the assay: CERK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CERK antibody and an CERK-HRP conjugate. The assay sample and buffer are incubated together with CERK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CERK concentration since CERK from samples and CERK-HRP conjugate compete for the anti-CERK antibody binding site. Since the number of sites is limited, as more sites are occupied by CERK from the sample, fewer sites are left to bind CERK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CERK concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CERK elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 20336726
• NCBI GeneID: 64781
• NCBI Accession #: NP_073603.2
• NCBI GenBank Nucleotide #: NM_022766.5
• Molecular Weight: 59,977 Da
• NCBI Official Full Name: ceramide kinase
• NCBI Official Synonym Full Names: ceramide kinase
• NCBI Official Symbol: CERK
• NCBI Official Synonym Symbols: LK4; hCERK; dA59H18.2; dA59H18.3
• NCBI Protein Information: ceramide kinase; lipid kinase 4; lipid kinase LK4; acylsphingosine kinase
• UniProt Protein Name: Ceramide kinase
• Protein Family: Ceramide kinase
• UniProt Gene Name: CERK
• UniProt Synonym Gene Names: KIAA1646; hCERK; LK4
• UniProt Entry Name: CERK1_HUMAN

NCBI Description

CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]).[supplied by OMIM, Mar 2008]

Uniprot Description

CERK: Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides.

Protein type: EC 2.7.1.138; Lipid Metabolism - sphingolipid; Kinase, lipid

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: mitochondrion; integral to membrane; plasma membrane

Molecular Function: ceramide kinase activity; magnesium ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: sphingolipid metabolic process; protein kinase C activation; glycosphingolipid metabolic process; ceramide metabolic process; lipid phosphorylation; transmembrane transport