Mouse Cellular Repressor Of E1A Stimulated Genes 1 (CREG1) ELISA Kit | CREG1 elisa kit

Mouse Cellular Repressor Of E1A Stimulated Genes 1 (CREG1) ELISA Kit

Cat. Num. AAA2023143

• Gene Names: CREG1; CREG
• Reactivity: Mouse
• Synonyms: Cellular Repressor Of E1A Stimulated Genes 1 (CREG1); Mouse Cellular Repressor Of E1A Stimulated Genes 1 (CREG1) ELISA Kit; CREG1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cellular Repressor Of E1A Stimulated Genes 1 (CREG1).; No significant cross-reactivity or interference between Cellular Repressor Of E1A Stimulated Genes 1 (CREG1) and analogues was observed.
• Sequence Length: 220

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.053ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cellular Repressor Of E1A Stimulated Genes 1 (CREG1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cellular Repressor Of E1A Stimulated Genes 1 (CREG1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CREG1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cellular Repressor Of E1A Stimulated Genes 1 (CREG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cellular Repressor Of E1A Stimulated Genes 1 (CREG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cellular Repressor Of E1A Stimulated Genes 1 (CREG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cellular Repressor Of E1A Stimulated Genes 1 (CREG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CREG1 elisa kit

CD & Adhesion molecule

NCBI and Uniprot Product Information

• NCBI GI #: 14250391
• NCBI GeneID: 8804
• UniProt Accession #: O75629; Q8N9A3; B2RDD4
• Molecular Weight: 24,075 Da
• NCBI Official Full Name: Cellular repressor of E1A-stimulated genes 1
• NCBI Official Synonym Full Names: cellular repressor of E1A-stimulated genes 1
• NCBI Official Symbol: CREG1
• NCBI Official Synonym Symbols: CREG
• NCBI Protein Information: protein CREG1
• UniProt Protein Name: Protein CREG1
• Protein Family: Protein
• UniProt Gene Name: CREG1
• UniProt Synonym Gene Names: CREG
• UniProt Entry Name: CREG1_HUMAN

NCBI Description

The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. The protein encoded by this gene antagonizes transcriptional activation and cellular transformation by E1A. This protein shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro. This gene may contribute to the transcriptional control of cell growth and differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

CREG1: May contribute to the transcriptional control of cell growth and differentiation. Antagonizes transcriptional activation and cellular transformation by the adenovirus E1A protein. The transcriptional control activity of cell growth requires interaction with IGF2R. Belongs to the CREG family.

Protein type: Secreted; Transcription, coactivator/corepressor; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q24

Cellular Component: transcription factor complex

Molecular Function: FMN binding; oxidoreductase activity; transcription corepressor activity; transcription factor binding

Biological Process: regulation of transcription from RNA polymerase II promoter; cell proliferation; regulation of growth; multicellular organismal development