Rabbit ERCC2 Polyclonal Antibody | anti-ERCC2 antibody

ERCC2 Polyclonal Antibody

Cat. Num. AAA8527368

• Gene Names: ERCC2; EM9; TTD; XPD; TTD1; COFS2; TFIIH
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: ERCC2; Polyclonal Antibody; ERCC2 Polyclonal Antibody; COFS2; EM9; MGC102762; MGC126218; MGC126219; TTD; XPD; anti-ERCC2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 760

• Applicable Applications for anti-ERCC2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:100
• Immunogen: Fusion protein of human ERCC2
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of HeLacell line, using ERCC2 antibody.)

Related Product Information for anti-ERCC2 antibody

XPB and XPD are ATPase/helicase subunits of the TFIIH complex that are involved in nucleotide excision repair (NER) to remove lesions and photoproducts generated by UV light (1). XPB and XPD are 3'-5' and 5'-3' DNA helicases, respectively, that play a role in opening of the DNA damage site to facilitate repair (2, 3). XPB and XPD both play an important role in maintaining genomic stability, and researchers have linked mutations of these proteins to XerodermaPigmentosum (XP) and Trichothiodystrophy (TTD). XP patients have abnormalities in skin pigmentation and are highly susceptible to skin cancers, while TTD patients exhibit symptoms such as brittle hair, neurological abnormalities, and mild photosensitivity (4). In addition to their role in NER, XPB and XPD are involved in transcription initiation as part of the TFIIH core complex (5). The helicase activity of XPB unwinds DNA around the transcription start site to facilitate RNA polymerase II promoter clearance and initiation of transcription (6). XPD plays a structural role linking core TFIIH components with the cdk-activating kinase (CAK) complex that phosphorylates the C-terminus of the largest subunit of RNA polymerase II, leading to transcription initiation (7).

References

1. Oksenych, V. and Coin, F. (2010) Cell Cycle 9, 90-6. 2. Evans, E. et al. (1997) EMBO J 16, 6559-73. 3. Riedl, T. et al. (2003) EMBO J 22, 5293-303. 4. Lehmann, A.R. (2003) Biochimie 85, 1101-11. 5. Drapkin, R. et al. (1994) Nature 368, 769-72. 6. Holstege, F.C. et al. (1996) EMBO J 15, 1666-77. 7. Rossignol, M. et al. (1997) EMBO J 16, 1628-37.

NCBI and Uniprot Product Information

• NCBI GI #: 15834617
• NCBI GeneID: 2068
• NCBI Accession #: NP_000391.1
• NCBI GenBank Nucleotide #: NM_000400.3
• UniProt Accession #: P18074; Q2TB78; Q2YDY2; Q7KZU6; Q8N721
• Molecular Weight: 87kDa
• NCBI Official Full Name: general transcription and DNA repair factor IIH helicase subunit XPD isoform 1
• NCBI Official Synonym Full Names: ERCC excision repair 2, TFIIH core complex helicase subunit
• NCBI Official Symbol: ERCC2
• NCBI Official Synonym Symbols: EM9; TTD; XPD; TTD1; COFS2; TFIIH
• NCBI Protein Information: general transcription and DNA repair factor IIH helicase subunit XPD
• UniProt Protein Name: General transcription and DNA repair factor IIH helicase subunit XPD
• Protein Family: TFIIH basal transcription factor complex helicase
• UniProt Gene Name: ERCC2
• UniProt Synonym Gene Names: XPD; XPDC; TFIIH subunit XPD; BTF2 p80; TFIIH 80 kDa subunit; TFIIH p80

NCBI Description

The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

ATP-dependent 5'-3' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers.