CD86 / B7-2 recombinant protein

Recombinant Mouse CD86 / B7-2 Protein (His tag)

Cat. Num. AAA2547241

• Gene Names: Cd86; B7; B70; MB7; B7-2; B7.2; CLS1; Ly58; ETC-1; Ly-58; MB7-2; Cd28l2; TS/A-2
• Purity: > 92 % as determined by SDS-PAGE
• Synonyms: CD86 / B7-2; Recombinant Mouse CD86 / B7-2 Protein (His tag); CD86; B7; B7-2; B7.2; B70; CLS1; Cd28l2; ETC-1; Ly-58; Ly58; MB7; MB7-2; TS/A-2; CD86 / B7-2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 92 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 309

• Application Notes: The recombinant mouse CD86 consists of 233 amino acids after removal of the signal peptide and has a predicted molecular mass of 26.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD86 is approximately 40-55 kDa due to glycosylation.
• Predicted N Terminal: Val 24
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD86 / B7-2 recombinant protein

Background: CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.

Description: A DNA sequence encoding the extracellular domain (Met 1-Glu 245) of mouse CD86 (NP_062261.3) precursor was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 161484599
• NCBI GeneID: 12524
• NCBI Accession #: NP_062261.3
• NCBI GenBank Nucleotide #: NM_019388.3
• UniProt Accession #: P42082
• Molecular Weight: 33,962 Da
• NCBI Official Full Name: T-lymphocyte activation antigen CD86
• NCBI Official Synonym Full Names: CD86 antigen
• NCBI Official Symbol: Cd86
• NCBI Official Synonym Symbols: B7; B70; MB7; B7-2; B7.2; CLS1; Ly58; ETC-1; Ly-58; MB7-2; Cd28l2; TS/A-2
• NCBI Protein Information: T-lymphocyte activation antigen CD86
• UniProt Protein Name: T-lymphocyte activation antigen CD86
• UniProt Gene Name: Cd86
• UniProt Synonym Gene Names: ETC-1

Uniprot Description

CD86: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16 B3|16 25.72 cM

Cellular Component: cell surface; external side of plasma membrane; intracellular membrane-bound organelle

Molecular Function: receptor binding

Biological Process: B cell activation; defense response to virus; negative regulation of T cell anergy; positive regulation of activated T cell proliferation; positive regulation of T cell proliferation; toll-like receptor 3 signaling pathway; toll-like receptor signaling pathway