Rat CD86 Monoclonal Antibody | anti-CD86 antibody

RAT ANTI MOUSE CD86:RPE

Cat. Num. AAA216137

• Gene Names: Cd86; B7; B70; MB7; B7-2; B7.2; CLS1; Ly58; ETC-1; Ly-58; MB7-2; Cd28l2; TS/A-2
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD86; Monoclonal Antibody; RAT ANTI MOUSE CD86:RPE; anti-CD86 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: PO3
• Form/Format: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
• Sequence Length: 309

• Applicable Applications for anti-CD86 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
• Reconstitution: Reconstitute in 0.25 ml disilled water; Perservative Stabilisers
• Immunogen: Mouse B-cell line, BCL1.; Fusion Partners
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data

(Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD86:FITC (MBS210978))

Related Product Information for anti-CD86 antibody

Rat anti mouse CD86 recognizes mouse CD86 (B7-2), an 80kD cell surface glycoprotein which is a member of the CD28/B7 family. In mouse CD86 is expressed at high levels on peripheral blood monocytes and dendritic cells, and at low levels on resting B and T-lymphocytes. Expression of CD86, on these cell populations, can be increased upon activation. CD86 has been identified, along with CD80 (B7-1), as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA4). CD28 and CTLA4 are two receptors that have opposing functions in T-cell stimulation. Interaction of CD86 with CD28 promotes a number of T-cell activities, whereas the binding of CD86 to CTLA4 inhibits T-cell responses. The PO3 antibody has been reported to block some co-stimulatory functions of CD86  (Nakajima et al. 1997).

NCBI and Uniprot Product Information

• NCBI GI #: 161484599
• NCBI GeneID: 12524
• NCBI Accession #: NP_062261.3
• NCBI GenBank Nucleotide #: NM_019388.3
• UniProt Accession #: P42082
• Molecular Weight: 33,962 Da
• NCBI Official Full Name: T-lymphocyte activation antigen CD86
• NCBI Official Synonym Full Names: CD86 antigen
• NCBI Official Symbol: Cd86
• NCBI Official Synonym Symbols: B7; B70; MB7; B7-2; B7.2; CLS1; Ly58; ETC-1; Ly-58; MB7-2; Cd28l2; TS/A-2
• NCBI Protein Information: T-lymphocyte activation antigen CD86; activation B7-2 antigen; early T cell costimulatory molecule-1; early T-cell costimulatory molecule 1
• UniProt Protein Name: T-lymphocyte activation antigen CD86
• Protein Family: T-lymphocyte activation antigen
• UniProt Gene Name: Cd86
• UniProt Synonym Gene Names: ETC-1
• UniProt Entry Name: CD86_MOUSE

Uniprot Description

CD86: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Cellular Component: cell surface; intracellular membrane-bound organelle; membrane; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: receptor binding

Biological Process: B cell activation; negative regulation of T cell anergy; immune system process; toll-like receptor signaling pathway; positive regulation of T cell proliferation; positive regulation of activated T cell proliferation; defense response to virus