Typical Testing Data/Standard Curve (for reference only)
(Mouse CCL3/MIP1 alpha PicoKine ELISA Kit standard curve )
Mouse CCL3/MIP1 alpha ELISA Kit | CCL3 elisa kit
Mouse CCL3/MIP1 alpha PicoKine ELISA Kit
Gene Names
Ccl3; Mip1a; Scya3; G0S19-1; AI323804; MIP1-(a); LD78alpha; MIP-1alpha; MIP1-alpha
Reactivity
Mouse
Synonyms
CCL3/MIP1 alpha; Mouse CCL3/MIP1 alpha PicoKine ELISA Kit; C-C motif chemokine 3; chemokine (C-C motif) ligand 3; C C motif chemokine 3; CCL3; CCL3_HUMAN; Chemokine (C C motif) ligand 3; Chemokine ligand 3; G0/G1 switch regulatory protein 19 1; G0/G1 switch regulatory protein 19-1; G0S19 1; G0S19 1 protein; Heparin binding chemotaxis protein; L2G25B; LD78-alpha(4-69); LD78ALPHA; Macrophage inflammatory protein 1 alpha; Macrophage inflammatory protein 1-alpha; MIP 1 alpha; MIP-1-alpha; MIP-1-alpha(4-69); MIP1A; PAT 464.1; SCYA 3; SCYA3; SIS alpha; SIS beta; SIS-beta; Small inducible cytokine A3; Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein; CCL3 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse CCL3
Sequence Length
92
Samples
Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Assay Type
Sandwich
Detection Range
7.8pg/ml-500pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse CCL3/MIP1 alpha PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse CCL3/MIP1 alpha PicoKine ELISA Kit standard curve )
Related Product Information for CCL3 elisa kit
Principle of the assay: mouse CCL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL3 has been precoated onto 96-well plates. Standards(E.coli, A24-A92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL3 amount of sample captured in plate.
Background: Chemokine(C-C motif) ligand 3(CCL3), so known as Macrophage inflammatory protein-1alpha(MIP-1alpha), is a protein that in humans is encoded by the CCL3 gene. It is a cytokine which belongs to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This gene is mapped to 17q12. CCL3 plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. It has been found that the activation of CCR5 by CCL3 directly and independently activates a G-protein signaling pathway through GNAI2 and a tyrosine phosphorylation signaling pathway through JAK2.
Background: Chemokine(C-C motif) ligand 3(CCL3), so known as Macrophage inflammatory protein-1alpha(MIP-1alpha), is a protein that in humans is encoded by the CCL3 gene. It is a cytokine which belongs to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This gene is mapped to 17q12. CCL3 plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. It has been found that the activation of CCR5 by CCL3 directly and independently activates a G-protein signaling pathway through GNAI2 and a tyrosine phosphorylation signaling pathway through JAK2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
10,345 Da
NCBI Official Full Name
C-C motif chemokine 3
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 3
NCBI Official Symbol
Ccl3
NCBI Official Synonym Symbols
Mip1a; Scya3; G0S19-1; AI323804; MIP1-(a); LD78alpha; MIP-1alpha; MIP1-alpha
NCBI Protein Information
C-C motif chemokine 3; TY-5; L2G25B; MIP1 (a); SIS-alpha; MIP-1 alpha; MIP-1-alpha; small inducible cytokine A3; small-inducible cytokine A3; heparin-binding chemotaxis protein; macrophage inflammatory protein-1alpha; macrophage inflammatory protein 1-alpha
UniProt Protein Name
C-C motif chemokine 3
Protein Family
UniProt Gene Name
Ccl3
UniProt Synonym Gene Names
Mip1a; Scya3; MIP-1-alpha
UniProt Entry Name
CCL3_MOUSE
Uniprot Description
CCL3L1: Chemotactic for lymphocytes and monocytes. Is a ligand for CCR1, CCR3 and CCR5. Is an inhibitor of HIV-1-infection. The processed form LD78-beta(3-70) shows a 20-fold to 30-fold higher chemotactic activity and is a very potent inhibitor of HIV-1- infection. LD78-beta(3-70) is also a ligand for CCR1, CCR3 and CCR5. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Secreted; Secreted, signal peptide; Chemokine; Motility/polarity/chemotaxis
Cellular Component: extracellular space; cell; extracellular region; cytosol
Molecular Function: protein binding; chemokine activity; cytokine activity
Biological Process: neutrophil chemotaxis; positive regulation of osteoclast differentiation; leukocyte chemotaxis; positive regulation of interleukin-1 beta secretion; positive regulation of tumor necrosis factor production; chemotaxis; macrophage chemotaxis; regulation of cell shape; elevation of cytosolic calcium ion concentration; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response; astrocyte cell migration; positive regulation of inflammatory response
Research Articles on CCL3
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Product Notes
The Mouse CCL3 ccl3 (Catalog #AAA177386) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177386 ELISA Kit recognizes Mouse CCL3. It is sometimes possible for the material contained within the vial of "CCL3/MIP1 alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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