Rabbit SHP-2 Polyclonal Antibody | anti-SHP-2 antibody

SHP-2 (Ab-542) Antibody

Cat. Num. AAA9407334

• Gene Names: PTPN11; CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Synonyms: SHP-2; Polyclonal Antibody; SHP-2 (Ab-542) Antibody; Protein-tyrosine phosphatase 2C; anti-SHP-2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total SHP-2 protein.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 593

• Applicable Applications for anti-SHP-2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100; Immunofluorescence: 1:100~1:200
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around aa. 540~544 (H-E-Y-T-N) derived from Human SHP-2.
• Target Name: SHP-2
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from 3T3 cells using SHP-2(Ab-542) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SHP-2(Ab-542) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells using SHP-2(Ab-542) Antibody.)

Related Product Information for anti-SHP-2 antibody

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.

Product Categories/Family for anti-SHP-2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 33356177
• NCBI GeneID: 5781
• NCBI Accession #: NP_002825.3
• NCBI GenBank Nucleotide #: NM_002834.3
• UniProt Accession #: Q06124; Q96HD7; A8K1D9
• Molecular Weight: 52,828 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 11 isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 11
• NCBI Official Symbol: PTPN11
• NCBI Official Synonym Symbols: CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 11
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 11
• UniProt Gene Name: PTPN11
• UniProt Synonym Gene Names: PTP2C; SHPTP2; PTP-1D; PTP-2C; SHP-2; Shp2
• UniProt Entry Name: PTN11_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

SHP-2: a SH2-containing a ubiquitously expressed tyrosine-specific protein phosphatase. It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens and extracellular matrices in the control of cell growth, differentiation, migration, and death. Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines.

Protein type: EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor); Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: protein complex; mitochondrion; cytoplasm; nucleus; cytosol

Molecular Function: protein domain specific binding; protein binding; phospholipase binding; peptide hormone receptor binding; SH3/SH2 adaptor activity; insulin receptor substrate binding; non-membrane spanning protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; D1 dopamine receptor binding; phosphoprotein phosphatase activity; insulin receptor binding

Biological Process: axon guidance; hormone-mediated signaling; nerve growth factor receptor signaling pathway; activation of MAPK activity; heart development; platelet-derived growth factor receptor signaling pathway; reproductive process in a multicellular organism; positive regulation of mitotic cell cycle; glucose homeostasis; negative regulation of insulin secretion; regulation of protein export from nucleus; regulation of cell adhesion mediated by integrin; ephrin receptor signaling pathway; abortive mitotic cell cycle; negative regulation of cell adhesion mediated by integrin; inner ear development; epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; negative regulation of growth hormone secretion; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; positive regulation of hormone secretion; regulation of multicellular organism growth; negative regulation of cortisol secretion; organ growth; genitalia development; triacylglycerol metabolic process; DNA damage checkpoint; T cell costimulation; insulin receptor signaling pathway; innate immune response; hormone metabolic process; brain development; platelet formation; blood coagulation; leukocyte migration

Disease: Metachondromatosis; Noonan Syndrome 1; Juvenile Myelomonocytic Leukemia; Leopard Syndrome 1