Mouse CCAAT/Enhancer Binding Protein Beta (CEBPb) ELISA Kit | CEBPb elisa kit

Mouse CCAAT/Enhancer Binding Protein Beta (CEBPb) ELISA Kit

Cat. Num. AAA2019139

• Gene Names: Cebpb; LAP; LIP; CRP2; NF-M; Nfil6; NF-IL6; IL-6DBP; C/EBPbeta
• Reactivity: Mouse
• Synonyms: CCAAT/Enhancer Binding Protein Beta (CEBPb); Mouse CCAAT/Enhancer Binding Protein Beta (CEBPb) ELISA Kit; CRP2; IL6DBP; LAP; NF-IL6; TCF5; C/EBP-Beta; Liver-Enriched Transcriptional Activator Protein; Nuclear Factor Of Interleukin 6; Interleukin 6-Dependent DNA-Binding Protein; CEBPb elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of CCAAT/Enhancer Binding Protein Beta (CEBPb).; No significant cross-reactivity or interference between CCAAT/Enhancer Binding Protein Beta (CEBPb) and analogues was observed.
• Sequence Length: 275

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 78.12-5000pg/mL
• Sensitivity: < 33pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CCAAT/Enhancer Binding Protein Beta (CEBPb) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CCAAT/Enhancer Binding Protein Beta (CEBPb) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CEBPb elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CCAAT/Enhancer Binding Protein Beta (CEBPb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CCAAT/Enhancer Binding Protein Beta (CEBPb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CCAAT/Enhancer Binding Protein Beta (CEBPb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CCAAT/Enhancer Binding Protein Beta (CEBPb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CEBPb elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 567757569
• NCBI GeneID: 12608
• NCBI Accession #: NP_001274667.1
• NCBI GenBank Nucleotide #: NM_001287738.1
• UniProt Accession #: P28033
• Molecular Weight: 15,597 Da
• NCBI Official Full Name: CCAAT/enhancer-binding protein beta isoform b
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein (C/EBP), beta
• NCBI Official Symbol: Cebpb
• NCBI Official Synonym Symbols: LAP; LIP; CRP2; NF-M; Nfil6; NF-IL6; IL-6DBP; C/EBPbeta
• NCBI Protein Information: CCAAT/enhancer-binding protein beta
• UniProt Protein Name: CCAAT/enhancer-binding protein beta
• Protein Family: CCAAT/enhancer-binding protein
• UniProt Gene Name: Cebpb
• UniProt Synonym Gene Names: C/EBP beta; IL-6DBP; LAP
• UniProt Entry Name: CEBPB_MOUSE

Uniprot Description

C/EBP-beta: a bZIP transcription factor which can form homodimers or heterodimers with the related proteins CEBP-alpha, -delta and -gamma. Involved in immune and inflammatory responses. Specifically binds to regulatory regions of genes encoding IL-6, other cytokines and several acute-phase proteins. There are two forms of C/EBPbeta, the 38kDa liver activating protein (LAP) and the 20kDa liver inhibitory protein (LIP) which may be products of alternative translation. LAP is a transcriptional activator while LIP may inhibit C/EBPbeta transcriptional activity. Phosphorylated and activated by ERK1/2.

Protein type: Transcription factor; Motility/polarity/chemotaxis; DNA-binding

Cellular Component: nuclear matrix; nuclear chromatin; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; chromatin binding; transcription factor binding; transcription factor activity; glucocorticoid receptor binding

Biological Process: transcription from RNA polymerase II promoter; fat cell differentiation; embryonic placenta development; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; regulation of interleukin-6 biosynthetic process; mammary gland epithelial cell proliferation; response to lipopolysaccharide; neuron differentiation; positive regulation of osteoblast differentiation; regulation of transcription, DNA-dependent; brown fat cell differentiation; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of neuron apoptosis; negative regulation of transcription, DNA-dependent; cell differentiation