Human Cathepsin Z (CTSZ) ELISA Kit | CTSZ elisa kit

Human Cathepsin Z (CTSZ) ELISA Kit

Cat. Num. AAA2023308

• Gene Names: CTSZ; CTSX
• Reactivity: Human
• Synonyms: Cathepsin Z (CTSZ); Human Cathepsin Z (CTSZ) ELISA Kit; CTS-Z; CTSX; Cathepsin P; Cathepsin X; CTSZ elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CTSZ. No significant cross-reactivity or interference between CTSZ and analogues was observed.
• Sequence Length: 303

• Samples: Human serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CTSZ were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CTSZ were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTSZ elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CTSZ. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CTSZ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CTSZ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CTSZ in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CTSZ elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 22538442
• NCBI GeneID: 1522
• NCBI Accession #: NP_001327.2
• NCBI GenBank Nucleotide #: NM_001336.3
• UniProt Accession #: Q9UBR2; O75331; Q9UQV5; Q9UQV6; B2RC40
• Molecular Weight: 33,868 Da
• NCBI Official Full Name: cathepsin Z preproprotein
• NCBI Official Synonym Full Names: cathepsin Z
• NCBI Official Symbol: CTSZ
• NCBI Official Synonym Symbols: CTSX
• NCBI Protein Information: cathepsin Z
• UniProt Protein Name: Cathepsin Z
• Protein Family: Cathepsin
• UniProt Gene Name: CTSZ
• UniProt Entry Name: CATZ_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal cysteine proteinase and member of the peptidase C1 family. It exhibits both carboxy-monopeptidase and carboxy-dipeptidase activities. The encoded protein has also been known as cathepsin X and cathepsin P. This gene is expressed ubiquitously in cancer cell lines and primary tumors and, like other members of this family, may be involved in tumorigenesis. [provided by RefSeq, Oct 2008]

Uniprot Description

CTSZ: Exhibits carboxy-monopeptidase as well as carboxy- dipeptidase activity. Belongs to the peptidase C1 family.

Protein type: Protease; EC 3.4.18.1

Chromosomal Location of Human Ortholog: 20q13.32

Cellular Component: extracellular space; intracellular membrane-bound organelle; lysosome; endoplasmic reticulum; plasma membrane

Molecular Function: cysteine-type peptidase activity

Biological Process: cellular protein metabolic process; angiotensin maturation; proteolysis

Disease: Mycobacterium Tuberculosis, Susceptibility To