Human Caspase 1 (CASP1) ELISA Kit | CASP1 elisa kit

Human Caspase 1 (CASP1) ELISA Kit

Cat. Num. AAA2024657

• Gene Names: Casp1; Ice; Il1bc
• Reactivity: Human
• Synonyms: Caspase 1 (CASP1); Human Caspase 1 (CASP1) ELISA Kit; ICE; IL1BC; IL1-BC; P45; Interleukin 1 Beta Converting Enzyme; Apoptosis-Related Cysteine Peptidase; Interleukin-1 beta convertase; CASP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Caspase 1 (CASP1).; No significant cross-reactivity or interference between Caspase 1 (CASP1) and analogues was observed.
• Sequence Length: 402

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.113ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 1 (CASP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 1 (CASP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CASP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 1 (CASP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 1 (CASP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 1 (CASP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 1 (CASP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CASP1 elisa kit

Signal transduction; Enzyme & Kinase; Apoptosis; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 31542341
• NCBI GeneID: 25166
• NCBI Accession #: NP_036894.2
• NCBI GenBank Nucleotide #: NM_012762.2
• UniProt Accession #: P43527
• Molecular Weight: 45,576 Da
• NCBI Official Full Name: caspase-1
• NCBI Official Synonym Full Names: caspase 1
• NCBI Official Symbol: Casp1
• NCBI Official Synonym Symbols: Ice; Il1bc
• NCBI Protein Information: caspase-1
• UniProt Protein Name: Caspase-1
• UniProt Gene Name: Casp1
• UniProt Synonym Gene Names: Il1bc; Il1bce; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme
• UniProt Entry Name: CASP1_RAT

NCBI Description

apoptotic protease that may play a role in inflammation and apoptosis in male sex organs [RGD, Feb 2006]

Uniprot Description

CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis (By similarity)

Protein type: Apoptosis; Protease; EC 3.4.22.36