SDS-PAGE
IGSF3 / EWI-3 recombinant protein
Recombinant Human IGSF3 / EWI-3 Protein (His tag)
Gene Names
IGSF3; V8; LCDD; EWI-3
Purity
> 98 % as determined by SDS-PAGE
Synonyms
IGSF3 / EWI-3; Recombinant Human IGSF3 / EWI-3 Protein (His tag); RP4-781D12.1; EWI-3; MGC117164; V8; IGSF3 / EWI-3 recombinant protein
Host
Human Cells
Purity/Purification
> 98 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
1194
Application Notes
The secreted recombinant human IGSF3 comprises 1116 amino acids and has a calculated molecular mass of 126.6 kDa. The apparent molecular mass of the recombinant protein is approximately 140 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Predicted N Terminal
Gln 20
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for IGSF3 / EWI-3 recombinant protein
Background: Immunoglobulin superfamily member 3 (IGSF3), also known as Glu-Trp-Ile EWI motif-containing protein 3, IgSF3 and EWI-3 and IGSF3, is a single-pass type I membrane protein which belongs to the EWI subfamily of the Ig Superfamily of molecules. IGSF3 has an overall structural similarity and strong sequence similarity to V7, a human leukocyte surface protein. IGSF3 contains eight Ig-like C2-type (immunoglobulin-like) domains. IGSF3 is expressed in a wide range of tissues with high expression in Placenta, kidney and lung. EWI-2 is part of a novel Ig subfamily that includes EWI-F (F2alpha receptor regulatory protein (FPRP), CD9P-1), EWI-3 (IGSF3), and EWI-101 (CD101). All four members of this Ig subfamily contain a Glu-Trp-Ile (EWI) motif not seen in other Ig proteins. Human IGSF3 shares 92 % aa identity with mouse IGSF3.
Description: A DNA sequence encoding the human IGSF3 isoform 1 (O75054-1) extracellular domain (Met 1-Ala 1124) was expressed, with a C-terminal polyhistidine tag.
Description: A DNA sequence encoding the human IGSF3 isoform 1 (O75054-1) extracellular domain (Met 1-Ala 1124) was expressed, with a C-terminal polyhistidine tag.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
137,516 Da
NCBI Official Full Name
immunoglobulin superfamily member 3 isoform 2
NCBI Official Synonym Full Names
immunoglobulin superfamily member 3
NCBI Official Symbol
IGSF3
NCBI Official Synonym Symbols
V8; LCDD; EWI-3
NCBI Protein Information
immunoglobulin superfamily member 3
UniProt Protein Name
Immunoglobulin superfamily member 3
UniProt Gene Name
IGSF3
UniProt Synonym Gene Names
EWI3; KIAA0466; IgSF3; EWI-3
NCBI Description
The protein encoded by this gene is an immunoglobulin-like membrane protein containing several V-type Ig-like domains. A mutation in this gene has been associated with bilateral nasolacrimal duct obstruction (LCDD). [provided by RefSeq, Jun 2016]
Uniprot Description
IGSF3: 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Immunoglobulin superfamily; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1p13.1
Cellular Component: cell surface; integral to membrane; plasma membrane
Biological Process: lacrimal gland development
Disease: Lacrimal Duct Defect
Research Articles on IGSF3 / EWI-3
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Product Notes
The IGSF3 / EWI-3 igsf3 (Catalog #AAA2545826) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The secreted recombinant human IGSF3 comprises 1116 amino acids and has a calculated molecular mass of 126.6 kDa. The apparent molecular mass of the recombinant protein is approximately 140 kDa in SDS-PAGE under reducing conditions due to glycosylation. Researchers should empirically determine the suitability of the IGSF3 / EWI-3 igsf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IGSF3 / EWI-3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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