Mouse C-telopeptide of type I collagen, CTX-I ELISA Kit | CTX-I elisa kit

Mouse C-telopeptide of type I collagen, CTX-I ELISA Kit

Cat. Num. AAA164705

• Reactivity: Mouse
• Synonyms: C-telopeptide of type I collagen; CTX-I; Mouse C-telopeptide of type I collagen; CTX-I ELISA Kit; CTX-I elisa kit

• Reactivity: Mouse
• Sequence Length: 81

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.05ng/ml - 15ng/ml
• Sensitivity: 0.01ng/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTX-I elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse C-telopeptide of Type I Collagen (also known as CTX- I) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse CTX- I antibody. CTX- I present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CTX- I Antibody is added and binds to CTX- I in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CTX- I antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse CTX- I. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 41688809
• UniProt Accession #: P60304; P01449; P01450; Q9PS24; Q9W6W8
• Molecular Weight: 8,992 Da
• NCBI Official Full Name: Cytotoxin 1
• UniProt Protein Name: Cytotoxin 1
• UniProt Gene Name: CTX A12 PublicationsManual assertion based on opinion iniRef.7
• UniProt Synonym Gene Names: CTX I

Uniprot Description

Basic protein that binds to cell membrane and depolarizes cardiomyocytes. It also shows lytic activities on many other cells, including red blood cells. Interaction with sulfatides in the cell membrane induces pore formation and cell internalization and is responsible for cytotoxicity in cardiomyocytes. It targets the mitochondrial membrane and induces mitochondrial swelling and fragmentation (). It binds to the integrin alpha-V/beta-3 (ITGAV/ITGB3) with a moderate affinity and inhibits protein kinases C (PubMed:8448165). It also binds with high affinity to heparin (PubMed:17685633). It also causes skeletal muscle necrosis after intramuscular injection into mice (PubMed:8342169).