Rat Cross Linked C-Telopeptide Of Type I Collagen (CTX-I) ELISA Kit | CTX-I elisa kit

Rat Cross Linked C-Telopeptide Of Type I Collagen (CTX-I) ELISA Kit

Cat. Num. AAA264213

• Reactivity: Rat
• Synonyms: Cross Linked C-Telopeptide Of Type I Collagen (CTX-I); Rat Cross Linked C-Telopeptide Of Type I Collagen (CTX-I) ELISA Kit; CTX-I elisa kit

• Reactivity: Rat
• Specificity: No cross-reaction with other factors.
• Sequence Length: 81

• Samples: Rat serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10000 pg/ml-156 pg/ml
• Sensitivity: Up to 50 pg/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTX-I elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat CTX-I monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 41688809
• Molecular Weight: 8,992 Da
• NCBI Official Full Name: Cytotoxin 1
• UniProt Protein Name: Cytotoxin 1
• UniProt Gene Name: CX1
• UniProt Synonym Gene Names: CTX-1; CTX1; CTX I; CTX-A1
• UniProt Entry Name: CTXA1_NAJAT

Uniprot Description

Basic protein that binds to cell membrane and depolarizes cardiomyocytes. It also shows lytic activities on many other cells, including red blood cells. Interaction with sulfatides in the cell membrane induces pore formation and cell internalization and is responsible for cytotoxicity in cardiomyocytes. It targets the mitochondrial membrane and induces mitochondrial swelling and fragmentation (). It binds to the integrin alpha-V/beta-3 (ITGAV/ITGB3) with a moderate affinity and inhibits protein kinases C (PubMed:8448165). It also binds with high affinity to heparin (PubMed:17685633). It also causes skeletal muscle necrosis after intramuscular injection into mice (PubMed:8342169).