Typical Standard Curve/Testing Data
Mouse Bradykinin (BK) ELISA Kit | BK elisa kit
Mouse Bradykinin (BK) Mini Samples ELISA Kit
Reactivity
Mouse
Synonyms
Bradykinin (BK); Mouse Bradykinin (BK) Mini Samples ELISA Kit; BK elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of bradykinin. No significant cross-reactivity or interference between bradykinin and analogues was observed.
Sequence Length
167
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type
Quantitative Competitive
Detection Range
24.69-2,000pg/mL
Sensitivity
Typically less than 9.21pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level bradykinin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level bradykinin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for BK elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of bradykinin in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to bradykinin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled bradykinin and unlabeled bradykinin (Standards or samples) with the pre-coated antibody specific to bradykinin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of bradykinin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of bradykinin in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to bradykinin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled bradykinin and unlabeled bradykinin (Standards or samples) with the pre-coated antibody specific to bradykinin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of bradykinin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of bradykinin in the sample.
Product Categories/Family for BK elisa kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
19,753 Da
NCBI Official Full Name
bradykinin
UniProt Protein Name
Kininogen-1
Uniprot Description
Vasodilator. Bradykinin produces in vitro relaxation of rat arterial smooth muscle and constriction of intestinal smooth muscle. May target bradykinin receptors (BDKRB).
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Product Notes
The Mouse BK (Catalog #AAA2087671) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2087671 ELISA Kit recognizes Mouse BK. It is sometimes possible for the material contained within the vial of "Bradykinin (BK), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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