Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Beta-Secretase (APH1B) Standard Curve.)
Mouse Beta-Secretase (APH1B) ELISA Kit | APH1B elisa kit
Mouse Beta-Secretase (APH1B) ELISA Kit
Reactivity
Mouse
Synonyms
Beta-Secretase (APH1B); Mouse Beta-Secretase (APH1B) ELISA Kit; APH1B; APH-1B; DKFZp564D0372; FLJ33115; PRO1328; PSFL; TAAV688; anterior pharynx defective 1B-like; presenilin stabilization factor-like; APH1B elisa kit
Reactivity
Mouse
Specificity
Mouse Beta-Secretase (APH1B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse APH1B. No significant cross-reactivity or interference between Mouse APH1B and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Beta-Secretase (APH1B) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Beta-Secretase (APH1B) Standard Curve.)
Related Product Information for APH1B elisa kit
Description: This Mouse Beta-Secretase (APH1B) ELISA Kit employs a two-site sandwich ELISA to quantitate APH1B in samples. An antibody specific for APH1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APH1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APH1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APH1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: APH1 is a multipass transmembrane protein that interacts with presenilin and nicastrin (APH2) as a functional component of the gamma-secretase complex. The gamma-secretase complex is required for the intramembrane proteolysis of a number of membrane proteins, including the amyloid-beta precursor protein and Notch.APH1B encodes a multi-pass transmembrane protein that is a functional component of the gamma-secretase complex, which also contains presenilin and nicastrin. This protein represents a stabilizing cofactor for the presenilin holoprotein in the complex. The gamma-secretase complex catalyzes the cleavage of integral proteins such as notch receptors and beta-amyloid precursor protein.
Background: APH1 is a multipass transmembrane protein that interacts with presenilin and nicastrin (APH2) as a functional component of the gamma-secretase complex. The gamma-secretase complex is required for the intramembrane proteolysis of a number of membrane proteins, including the amyloid-beta precursor protein and Notch.APH1B encodes a multi-pass transmembrane protein that is a functional component of the gamma-secretase complex, which also contains presenilin and nicastrin. This protein represents a stabilizing cofactor for the presenilin holoprotein in the complex. The gamma-secretase complex catalyzes the cleavage of integral proteins such as notch receptors and beta-amyloid precursor protein.
Similar Products
Product Notes
The Mouse APH1B (Catalog #AAA9718147) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718147 ELISA Kit recognizes Mouse APH1B. It is sometimes possible for the material contained within the vial of "Beta-Secretase (APH1B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
