Typical Testing Data/Standard Curve (for reference only)
(Mouse BCAM PicoKine ELISA Kit standard curve )
Mouse BCAM ELISA Kit | BCAM elisa kit
Mouse BCAM PicoKine ELISA Kit
Gene Names
Bcam; Lu; Gplu; B-CAM; 1200005K12Rik
Reactivity
Mouse
Synonyms
BCAM; Mouse BCAM PicoKine ELISA Kit; AU; Bcam; CD239; CD239 antigen; F8/G253 antigen; LU; Lutheran; MSK19; Q9R069; Basal cell adhesion molecule; basal cell adhesion molecule (Lutheran blood group); BCAM elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse BCAM
Sequence Length
622
Samples
Cell culture supernates, serum and plasma(heparin, EDTA).
Assay Type
Sandwich
Detection Range
156pg/ml-10000pg/ml
Sensitivity
<15pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
Typical Testing Data/Standard Curve (for reference only)
(Mouse BCAM PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse BCAM PicoKine ELISA Kit standard curve )
Related Product Information for BCAM elisa kit
Principle of the Assay: Mouse BCAM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BCAM has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: E26-A541) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BCAM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin- Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse BCAM amount of sample captured in plate.
Background/Introduction: Basal cell adhesion molecule is a protein that in humans is encoded by the BCAM gene. The BCAM gene encodes a glycoprotein expressed on cell surfaces. It is a member of the immunoglobulin superfamily of receptors and cell adhesion molecules. When expressed on erythrocytes, BCAM constitutes the Lutheran (Lu) and Auberger (Au) blood group antigens. And this protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene.
Background/Introduction: Basal cell adhesion molecule is a protein that in humans is encoded by the BCAM gene. The BCAM gene encodes a glycoprotein expressed on cell surfaces. It is a member of the immunoglobulin superfamily of receptors and cell adhesion molecules. When expressed on erythrocytes, BCAM constitutes the Lutheran (Lu) and Auberger (Au) blood group antigens. And this protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67,670 Da
NCBI Official Full Name
basal cell adhesion molecule
NCBI Official Synonym Full Names
basal cell adhesion molecule
NCBI Official Symbol
Bcam
NCBI Official Synonym Symbols
Lu; Gplu; B-CAM; 1200005K12Rik
NCBI Protein Information
basal cell adhesion molecule
UniProt Protein Name
Basal cell adhesion molecule
Protein Family
UniProt Gene Name
Bcam
UniProt Synonym Gene Names
Gplu; Lu
UniProt Entry Name
BCAM_MOUSE
Uniprot Description
Lu: Laminin alpha-5 receptor. May mediate intracellular signaling.
Protein type: Receptor, misc.; Membrane protein, integral
Cellular Component: cell surface; integral to plasma membrane; plasma membrane
Molecular Function: laminin binding; laminin receptor activity; protein C-terminus binding
Biological Process: cell adhesion; cell-matrix adhesion
Research Articles on BCAM
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Product Notes
The Mouse BCAM bcam (Catalog #AAA178576) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178576 ELISA Kit recognizes Mouse BCAM. It is sometimes possible for the material contained within the vial of "BCAM, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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