Mouse Basic leucine zipper transcriptional factor ATF-like 2 (BATF2) ELISA Kit | BATF2 elisa kit
Mouse Basic leucine zipper transcriptional factor ATF-like 2 (BATF2) ELISA Kit
Principle of the Assay: BATF2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BATF2 antibody and an BATF2-HRP conjugate. The assay sample and buffer are incubated together with BATF2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BATF2 concentration since BATF2 from samples and BATF2-HRP conjugate compete for the anti-BATF2 antibody binding site. Since the number of sites is limited, as more sites are occupied by BATF2 from the sample, fewer sites are left to bind BATF2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BATF2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
BATF2: Belongs to the bZIP family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 11q13.1
Cellular Component: nucleus
Molecular Function: protein binding; sequence-specific DNA binding; transcription factor activity
Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; myeloid dendritic cell differentiation; defense response to protozoan
Research Articles on BATF2
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Product Notes
The Mouse BATF2 batf2 (Catalog #AAA7223550) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7223550 ELISA Kit recognizes Mouse BATF2. It is sometimes possible for the material contained within the vial of "Basic leucine zipper transcriptional factor ATF-like 2 (BATF2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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