Immunohistochemistry (IHC)
(Staining of mouse heart. The antibody stained segments of muscle fibers in the atrium (paraffin sections treated with trypsin and with SDS prior to incubuation with the MBS618317).)
Muscarinic Receptor M2, aa225-356 Polyclonal Antibody | anti-CHRM2 antibody
Muscarinic Receptor M2, aa225-356 (Cholinergic Muscarinic Receptor M2, Chrm2, Muscarinic Receptor M2)
Gene Names
CHRM2; HM2; FLJ43243; MGC120006; MGC120007; CHRM2
Reactivity
Canine, Human, Mouse, Porcine, Rat
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Muscarinic Receptor M2; aa225-356; Polyclonal Antibody; aa225-356 (Cholinergic Muscarinic Receptor M2; Chrm2; Muscarinic Receptor M2); Anti -Muscarinic Receptor M2; anti-CHRM2 antibody
Host
Host: Rabbit; Source: Human
Reactivity
Canine, Human, Mouse, Porcine, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Muscarinic Receptor M2. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS, pH 7.4, 1% BSA, 5% sucrose, 0.05% sodium azide. Reconstitution: Reconstitute with 50ul sterile, 40-50% glycerol for long term storage at -20 degree C
Sequence
VANQD PVSPS LVQGR IVKPN NNNMP SSDDG LEHNK IQNGK APRDP VTENC VQGEE KESSN DSTSV SAVAS NMRDD EITQD ENTVS TSLGH SKDEN SKQTC IRIGT KTPKS DSCTP TNTTV EVVGS SGQNG DE
Applicable Applications for anti-CHRM2 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
Dilution: Western Blot: 1:200 (Rat brain membranes)
Immunohistochemistry: Rat brain sections
Dilution: Western Blot: 1:200 (Rat brain membranes)
Immunohistochemistry: Rat brain sections
Immunogen
GST fusion protein with a sequence of human m2 corresponding to aa225-356 (Accession P08172).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. May be stored at 4 degree C for short-term only. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunohistochemistry (IHC)
(Staining of mouse heart. The antibody stained segments of muscle fibers in the atrium (paraffin sections treated with trypsin and with SDS prior to incubuation with the MBS618317).)
Immunohistochemistry (IHC)
(Staining of mouse heart. The antibody stained segments of muscle fibers in the atrium (paraffin sections treated with trypsin and with SDS prior to incubuation with the MBS618317).)
Testing Data
(MBS618317 staining of rat hippocampus. There is selective staining around pyramidal cells in CA1-3 and around granule cells in the dentate gyrus. There is also diffuse staining in the striatum radiatum which is more extensive medially and becomes a thin layer running through CA3 toward the dentate gyrus.)
Testing Data
(MBS618317 staining of rat hippocampus. There is selective staining around pyramidal cells in CA1-3 and around granule cells in the dentate gyrus. There is also diffuse staining in the striatum radiatum which is more extensive medially and becomes a thin layer running through CA3 toward the dentate gyrus.)
Product Categories/Family for anti-CHRM2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
muscarinic acetylcholine receptor M2 [Homo sapiens]
NCBI Official Synonym Full Names
cholinergic receptor, muscarinic 2
NCBI Official Symbol
CHRM2
NCBI Official Synonym Symbols
HM2; FLJ43243; MGC120006; MGC120007; CHRM2
NCBI Protein Information
muscarinic acetylcholine receptor M2; 7TM receptor; OTTHUMP00000208394; OTTHUMP00000208395; muscarinic M2 receptor; cholinergic receptor, muscarinic 2, isoform a
UniProt Protein Name
Muscarinic acetylcholine receptor M2
Protein Family
UniProt Gene Name
CHRM2
UniProt Entry Name
ACM2_HUMAN
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Product Notes
The CHRM2 chrm2 (Catalog #AAA618317) is an Antibody produced from Host: Rabbit; Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Muscarinic Receptor M2, aa225-356 (Cholinergic Muscarinic Receptor M2, Chrm2, Muscarinic Receptor M2) reacts with Canine, Human, Mouse, Porcine, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Muscarinic Receptor M2, aa225-356 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Dilution: Western Blot: 1:200 (Rat brain membranes) Immunohistochemistry: Rat brain sections. Researchers should empirically determine the suitability of the CHRM2 chrm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: VANQD PVSPS LVQGR IVKPN NNNMP SSDDG LEHNK IQNGK APRDP VTENC VQGEE KESSN DSTSV SAVAS NMRDD EITQD ENTVS TSLGH SKDEN SKQTC IRIGT KTPKS DSCTP TNTTV EVVGS SGQNG DE. It is sometimes possible for the material contained within the vial of "Muscarinic Receptor M2, aa225-356, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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