Typical Testing Data/Standard Curve (for reference only)
Mouse Aspartate aminotransferase, cytoplasmic ELISA Kit | Got1 elisa kit
Mouse Aspartate aminotransferase, cytoplasmic ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aspartate aminotransferase, cytoplasmic. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Aspartate aminotransferase, cytoplasmic and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Aspartate aminotransferase, cytoplasmic, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Aspartate aminotransferase, cytoplasmic in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Protein type: Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - phenylalanine; EC 2.6.1.3; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.1; Transferase
Cellular Component: cytoplasm; cytosol; lysosome; nerve terminal; nucleus
Molecular Function: aspartate transaminase activity; carboxylic acid binding; catalytic activity; cysteine transaminase activity; identical protein binding; phosphatidylserine decarboxylase activity; pyridoxal phosphate binding; transaminase activity; transferase activity
Biological Process: amino acid biosynthetic process; amino acid metabolic process; aspartate biosynthetic process; aspartate catabolic process; aspartate metabolic process; biosynthetic process; dicarboxylic acid metabolic process; fatty acid homeostasis; glutamate catabolic process to 2-oxoglutarate; glutamate catabolic process to aspartate; glycerol biosynthetic process; Notch signaling pathway; oxaloacetate metabolic process
Research Articles on Got1
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Product Notes
The Mouse Got1 got1 (Catalog #AAA2886080) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2886080 ELISA Kit recognizes Mouse Got1. It is sometimes possible for the material contained within the vial of "Aspartate aminotransferase, cytoplasmic, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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