Mouse Arginase-1 (ARG1) ELISA Kit | ARG1 elisa kit

Mouse Arginase-1 (ARG1) ELISA Kit

Cat. Num. AAA9718015

• Gene Names: Arg1; AI; PGIF; Arg-1; AI256583
• Reactivity: Mouse
• Synonyms: Arginase-1 (ARG1); Mouse Arginase-1 (ARG1) ELISA Kit; ARG1; Arginase 1; liver-type arginase; type I arginase; ARG1 elisa kit

• Reactivity: Mouse
• Specificity: Mouse Arginase-1 (ARG1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ARG1. No significant cross-reactivity or interference between Mouse ARG1 and analogues was observed.
• Sequence Length: 323

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Arginase-1 (ARG1) Standard Curve.)

Related Product Information for ARG1 elisa kit

Description: This Mouse Arginase-1 (ARG1) ELISA Kit employs a two-site sandwich ELISA to quantitate ARG1 in samples. An antibody specific for ARG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ARG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ARG1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ARG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Arginase is the fifth and final step in the urea cycle, a series of biophysical reactions in mammals during which the body disposes of harmful ammonia. Specifically, arginase converts L-arginine into L-ornithine and urea. In most mammals, two isozymes of this enzyme exist; the first, Arginase I, functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate. It may be found at lower levels in macrophages, lactating mammary glands, and brain. The second isozyme may be found in the absence of other urea cycle enzymes. Arginase consists of three tetramers.

NCBI and Uniprot Product Information

• NCBI GI #: 7106255
• NCBI GeneID: 11846
• NCBI Accession #: NP_031508.1
• NCBI GenBank Nucleotide #: NP_031508.1
• UniProt Accession #: Q61176
• NCBI Official Full Name: arginase-1
• NCBI Official Synonym Full Names: arginase, liver
• NCBI Official Symbol: Arg1
• NCBI Official Synonym Symbols: AI; PGIF; Arg-1; AI256583
• NCBI Protein Information: arginase-1
• UniProt Protein Name: Arginase-1
• Protein Family: Arginase
• UniProt Gene Name: Arg1

Uniprot Description

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.