Mouse Aquaporin 3, Gill Blood Group (AQP3) ELISA Kit | AQP3 elisa kit

Mouse Aquaporin 3, Gill Blood Group (AQP3) ELISA Kit

Cat. Num. AAA452788

• Gene Names: Aqp3; AQP-2
• Reactivity: Mouse
• Synonyms: Aquaporin 3; Gill Blood Group (AQP3); Mouse Aquaporin 3; Gill Blood Group (AQP3) ELISA Kit; GIL; Aquaglyceroporin-3; AQP3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of AQP3. No significant cross-reactivity or interference between AQP3 and analogues was observed.
• Sequence Length: 292

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.058ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AQP3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AQP3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AQP3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of AQP3 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AQP3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to AQP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AQP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of AQP3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 121949813
• NCBI GeneID: 11828
• NCBI Accession #: NP_057898.2
• NCBI GenBank Nucleotide #: NM_016689.2
• UniProt Accession #: Q8R2N1; Q3TU75; Q9JJN8; Q9WTJ3
• Molecular Weight: 31,568 Da
• NCBI Official Full Name: aquaporin-3
• NCBI Official Synonym Full Names: aquaporin 3
• NCBI Official Symbol: Aqp3
• NCBI Official Synonym Symbols: AQP-2
• NCBI Protein Information: aquaporin-3
• UniProt Protein Name: Aquaporin-3
• Protein Family: Aquaporin
• UniProt Gene Name: Aqp3
• UniProt Synonym Gene Names: AQP-3

Uniprot Description

Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism.