Human Aquaporin 3, Gill Blood Group (AQP3) RTU ELISA Kit | AQP3 rtu elisa kit

Human Aquaporin 3, Gill Blood Group (AQP3) ELISA Kit

Cat. Num. AAA457739

• Gene Names: AQP3; GIL; AQP-3
• Reactivity: Human
• Synonyms: Aquaporin 3; Gill Blood Group (AQP3); Human Aquaporin 3; Gill Blood Group (AQP3) ELISA Kit; GIL; Aquaglyceroporin-3; AQP3 rtu elisa kit

• Reactivity: Human
• Sequence Length: 237

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.126ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AQP3 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AQP3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to AQP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AQP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AQP3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 970259826
• NCBI GeneID: 360
• NCBI Accession #: NP_001305073.1
• NCBI GenBank Nucleotide #: NM_001318144.1
• UniProt Accession #: Q92482; O00108; Q6FGT2; Q6FGW6; A8K843; B2RE16; D3DRL3
• Molecular Weight: 29,987 Da
• NCBI Official Full Name: aquaporin-3 isoform 2
• NCBI Official Synonym Full Names: aquaporin 3 (Gill blood group)
• NCBI Official Symbol: AQP3
• NCBI Official Synonym Symbols: GIL; AQP-3
• NCBI Protein Information: aquaporin-3
• UniProt Protein Name: Aquaporin-3
• Protein Family: Aquaporin
• UniProt Gene Name: AQP3
• UniProt Synonym Gene Names: AQP-3

NCBI Description

This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

Uniprot Description

Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism ().