Typical Testing Data/Standard Curve (for reference only)
Mouse Apolipoprotein H (APOH) ELISA Kit | APOH elisa kit
Mouse Apolipoprotein H (APOH) ELISA Kit
Reactivity
Mouse
Synonyms
Apolipoprotein H (APOH); Mouse Apolipoprotein H (APOH) ELISA Kit; APOH elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Apolipoprotein H (APOH)
No significant cross-reactivity or interference between Apolipoprotein H (APOH) and analogues was observed.
No significant cross-reactivity or interference between Apolipoprotein H (APOH) and analogues was observed.
Sequence Length
345
Samples
Serum, plasma and other biological fluids
Assay Type
Competitive
Detection Range
6.17-500ng/mL The standard curve concentrations used for the ELISA's were 500ng/mL, 166.67ng/mL, 55.56ng/mL, 18.52ng/mL, 6.17ng/mL
Sensitivity
Typically less than 2.91ng/mL.
Organism Species
Mus musculus (Mouse)
ELISA Type
Competitive
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein H (APOH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein H (APOH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein H (APOH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for APOH elisa kit
This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific for Apolipoprotein H (APOH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Apolipoprotein H (APOH) and unlabeled Apolipoprotein H (APOH) (Standards or samples) with the pre-coated antibody specific for Apolipoprotein H (APOH). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Apolipoprotein H (APOH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Apolipoprotein H (APOH) in the sample.
NCBI and Uniprot Product Information
Uniprot Description
Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.
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Product Notes
The Mouse APOH (Catalog #AAA2019999) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2019999 ELISA Kit recognizes Mouse APOH. It is sometimes possible for the material contained within the vial of "Apolipoprotein H (APOH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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