Mouse ANGPTL7 ELISA Kit | ANGPTL7 elisa kit

Mouse ANGPTL7 PicoKine ELISA Kit

Cat. Num. AAA1752641

• Reactivity: Mouse
• Synonyms: ANGPTL7; Mouse ANGPTL7 PicoKine ELISA Kit; Mouse ANGPTL7; angiopoietin-like 7; Angiopoietin-related protein 7; Angiopoietin-like protein 7; Angptl7; ANGPTL7 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Angptl7
• Sequence Length: 337

• Samples: Cell culture supernatants, serum and plasma (heparin, edta)
• Detection Range: 93.7 pg/ml - 6,000 pg/ml
• Sensitivity: <50 pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for ANGPTL7 elisa kit

Principle of the Assay: The Picokine™ Mouse Angptl7 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Angptl7 with a 96-well strip plate that is pre-coated with antibody specific for Angptl7. The detection antibody is a biotinylated antibody specific for Angptl7. The capture antibody is a polyclonal antibody from goat and the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Mouse Angptl7 with immunogen: Expression system for standard: CHO; Immunogen sequence: Q22-P337. The kit is analytically validated with ready-to-use reagents. To measure Mouse Angptl7, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Angptl7 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Angptl7 in the sample.

Background/Introduction: Angiopoietin-related protein 7 is a protein that in humans is encoded by the ANGPTL7 gene. This gene is mapped to 4; 4 E2. Angiopoietin-like Protein 7 (ANGPTL7), also known as cornea-derived transcript 6 (CDT6), is a secreted 45 kDa protein that contains a coiled-coil domain (aa 30?110) and a fibrinogen-like domain (aa 113?334). ANGPTL7 is expressed in the stromal layer of the cornea, and it forms disulfide-linked homotetramers. It contributes to corneal morphogenesis by suppressing angiogenesis and promoting the deposition of extracellular matrix components. ANGPTL7 is up-regulated in glaucoma and accumulates in the aqueous humor. Mature mouse ANGPTL7 shares 88% and 98% sequence identity with human and rat ANGPTL7.

NCBI and Uniprot Product Information

• NCBI GI #: 88196773
• NCBI GeneID: 654812
• NCBI Accession #: NP_001034643.1
• NCBI GenBank Nucleotide #: NP_001034643.1
• UniProt Accession #: Q8R1Q3
• NCBI Official Full Name: angiopoietin-related protein 7
• NCBI Official Synonym Full Names: angiopoietin-like 7
• NCBI Official Symbol: Angptl7
• NCBI Protein Information: angiopoietin-related protein 7
• UniProt Protein Name: Angiopoietin-related protein 7
• Protein Family: Angiopoietin-related protein
• UniProt Gene Name: Angptl7
• UniProt Entry Name: ANGL7_MOUSE

Uniprot Description

ANGPTL7:

Protein type: Secreted; Secreted, signal peptide