Mouse Angiotension I (ANG-1) ELISA Kit | ANG-1 elisa kit

Mouse Angiotension I (ANG-1) ELISA Kit

Cat. Num. AAA9718172

• Gene Names: ANGPT1; AGP1; AGPT; ANG1
• Reactivity: Mouse
• Synonyms: Angiotension I (ANG-1); Mouse Angiotension I (ANG-1) ELISA Kit; ANG-1 elisa kit

• Reactivity: Mouse
• Specificity: Mouse Angiotension I (ANG-1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ANG-1. No significant cross-reactivity or interference between Mouse ANG-1 and analogues was observed.
• Sequence Length: 497

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Angiotension I (ANG-1) Standard Curve.)

Related Product Information for ANG-1 elisa kit

Description: This Mouse Angiotension I (ANG-1) ELISA Kit employs a two-site sandwich ELISA to quantitate ANG-1 in samples. An antibody specific for ANG-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANG-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANG-1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANG-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Angiotensin I is formed by the action of renin on angiotensinogen. Renin cleaves the peptide bond between the leucine (Leu) and valine (Val) residues on angiotensinogen, creating the ten-amino acid peptide (des-Asp) angiotensin I. Renin is produced in the kidneys in response to renal sympathetic activity, decreased intrarenal blood pressure (<90mmHg systolic blood pressure) at the juxtaglomerular cells, or decreased delivery of Na+ and Cl- to the macula densa. If a reduced NaCl concentration in the distal tubule is sensed by the macula densa, renin release by juxtaglomerular cells is increased. This sensing mechanism for macula densa-mediated renin secretion appears to have a specific dependency on chloride ions rather than sodium ions. Studies using isolated preparations of thick ascending limb with glomerulus attached in low NaCl perfusate were unable to inhibit renin secretion when various sodium salts were added but could inhibit renin secretion with the addition of chloride salts.

NCBI and Uniprot Product Information

• NCBI GI #: 315434240
• NCBI GeneID: 284
• NCBI GenBank Nucleotide #: NP_001186788.1
• UniProt Accession #: Q15389
• NCBI Official Full Name: angiopoietin-1 isoform 2
• NCBI Official Synonym Full Names: angiopoietin 1
• NCBI Official Symbol: ANGPT1
• NCBI Official Synonym Symbols: AGP1; AGPT; ANG1
• NCBI Protein Information: angiopoietin-1

NCBI Description

This gene encodes a secreted glycoprotein that belongs to the angiopoietin family. Members of this family play important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]