Rabbit anti-Human, Mouse CD10 Polyclonal Antibody | anti-CD10 antibody

CD10, CT (Atriopeptidase, CALLA, CD 10, Common Acute Lymphocytic Leukemia Antigen, Enkephalinase, EPN, gp100, Membrane Metalloendopeptidase, MME, Neprilysin, NEP, Neutral Endopeptidase 24.11, Pmel17) (PE)

Cat. Num. AAA6467994

• Gene Names: MME; NEP; SFE; CD10; CALLA; CMT2T; SCA43
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CD10; Polyclonal Antibody; CT (Atriopeptidase; CALLA; CD 10; Common Acute Lymphocytic Leukemia Antigen; Enkephalinase; EPN; gp100; Membrane Metalloendopeptidase; MME; Neprilysin; NEP; Neutral Endopeptidase 24.11; Pmel17) (PE); DKFZp686O16152; EC=3.4.24.11; MGC126681; MGC126707; Neutral Endopeptidase; anti-CD10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MME. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CD10 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human MME (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Neprilysin antibody (Center) in mouse kidney tissue lysates (35ug/lane). Neprilysin (arrow) was detected using the purified Pab.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of MME Antibody (Center) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-CD10 antibody

Antibodies; CD Markers

References

1. Dakka, N., Bellaoui, H. Pediatr Hematol Oncol 26(4), 216-231 (2009). 2. Wang, R., Wang, S. J. Neurochem. 108(4), 1072-1082 (2009). 3. Shipp, M.A. Proc. Natl. Acad. Sci. USA 88(23), 10662-10666 (1991). 4. Shipp, M.A. Proc. Natl. Acad. Sci. USA 86(1), 297-301 (1989).

NCBI and Uniprot Product Information

• NCBI GI #: 116256329
• NCBI GeneID: 4311
• NCBI Accession #: NP_000893
• NCBI GenBank Nucleotide #: NM_000902.3
• UniProt Accession #: P08473; Q3MIX4; A8K6U6; D3DNJ9
• Molecular Weight: 80.9kDa (708aa) 70-100kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: neprilysin isoform a
• NCBI Official Synonym Full Names: membrane metalloendopeptidase
• NCBI Official Symbol: MME
• NCBI Official Synonym Symbols: NEP; SFE; CD10; CALLA; CMT2T; SCA43
• NCBI Protein Information: neprilysin
• UniProt Protein Name: Neprilysin
• Protein Family: CD109 antigen
• UniProt Gene Name: MME
• UniProt Synonym Gene Names: EPN; CALLA; NEP; Neutral endopeptidase; SFE
• UniProt Entry Name: NEP_HUMAN

NCBI Description

The protein encoded by this gene is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. [provided by RefSeq, Aug 2017]