Human Renin Precursor (RENP) ELISA Kit | RENP elisa kit

Human Renin Precursor (RENP) ELISA Kit

Cat. Num. AAA9714472

• Gene Names: REN; HNFJ2
• Reactivity: Human
• Synonyms: Renin Precursor (RENP); Human Renin Precursor (RENP) ELISA Kit; RENP elisa kit

• Reactivity: Human
• Specificity: Human Renin precursor (RENP) ELISA Kit has high sensitivity and excellent specificity for detection of Human RENP. No significant cross-reactivity or interference between Human RENP and analogues was observed.
• Sequence Length: 406

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Renin precursor (RENP) Standard Curve.)

Related Product Information for RENP elisa kit

Description: This Human Renin precursor (RENP) ELISA Kit employs a two-site sandwich ELISA to quantitate RENP in samples. An antibody specific for RENP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RENP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RENP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RENP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: The REN gene provides instructions for making a protein called renin, which is produced in the kidneys. This protein is part of the renin-angiotensin system, which regulates blood pressure and the balance of fluids and salts in the body. In the first step of this process, renin converts a protein called angiotensinogen into angiotensin I. Through an additional step, angiotensin I is converted to angiotensin II. Angiotensin II causes blood vessels to narrow (constrict), which results in increased blood pressure. Angiotensin II also stimulates production of the hormone aldosterone, which triggers the absorption of water and salt by the kidneys. The increased amount of fluid in the body also increases blood pressure. Proper blood pressure during fetal growth, which delivers oxygen to the developing tissues, is required for normal development of the kidneys, particularly of structures called the proximal tubules, and other tissues. In addition, angiotensin II may play a more direct role in kidney development, perhaps by affecting growth factors involved in development of kidney structures.

NCBI and Uniprot Product Information

• NCBI GI #: 4506475
• NCBI GeneID: 5972
• NCBI GenBank Nucleotide #: NP_000528.1
• UniProt Accession #: P00797
• NCBI Official Full Name: renin preproprotein
• NCBI Official Synonym Full Names: renin
• NCBI Official Symbol: REN
• NCBI Official Synonym Symbols: HNFJ2
• NCBI Protein Information: renin
• UniProt Protein Name: Renin
• UniProt Gene Name: REN
• UniProt Entry Name: RENI_HUMAN

NCBI Description

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq, Jul 2008]

Uniprot Description

REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.23.15; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular space; cytoplasm; plasma membrane; extracellular region

Molecular Function: peptidase activity; insulin-like growth factor receptor binding; aspartic-type endopeptidase activity; receptor binding

Biological Process: response to cAMP; hormone-mediated signaling; drinking behavior; male gonad development; renin-angiotensin regulation of aldosterone production; cell maturation; response to lipopolysaccharide; angiotensin maturation; proteolysis; cellular protein metabolic process; regulation of blood pressure; regulation of MAPKKK cascade; beta-amyloid metabolic process; mesonephros development; kidney development

Disease: Renal Tubular Dysgenesis; Hyperuricemic Nephropathy, Familial Juvenile, 2