Mouse Angiopoietin-like protein 3, ANGPTL3 ELISA Kit | ANGPTL3 elisa kit

Mouse Angiopoietin-like protein 3, ANGPTL3 ELISA Kit

Cat. Num. AAA166272

• Gene Names: ANGPTL3; ANL3; ANG-5; FHBL2; ANGPT5
• Reactivity: Mouse
• Synonyms: Angiopoietin-like protein 3; ANGPTL3; Mouse Angiopoietin-like protein 3; ANGPTL3 ELISA Kit; ANGPTL3 elisa kit

• Reactivity: Mouse
• Sequence Length: 460

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 10ng/ml - 3000ng/ml
• Sensitivity: 5.62ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANGPTL3 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Angiopoietin Like Protein 3 (also known as ANGPTL3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse ANGPTL3 antibody. ANGPTL3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ANGPTL3 Antibody is added and binds to ANGPTL3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ANGPTL3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ANGPTL3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 7656888
• NCBI GeneID: 27329
• NCBI Accession #: NP_055310.1
• NCBI GenBank Nucleotide #: NM_014495.3
• UniProt Accession #: Q9Y5C1; A0JLS0; B1ALJ0; B2RCW1
• Molecular Weight: 53,637 Da
• NCBI Official Full Name: angiopoietin-related protein 3 preproprotein
• NCBI Official Synonym Full Names: angiopoietin like 3
• NCBI Official Symbol: ANGPTL3
• NCBI Official Synonym Symbols: ANL3; ANG-5; FHBL2; ANGPT5
• NCBI Protein Information: angiopoietin-related protein 3
• UniProt Protein Name: Angiopoietin-related protein 3
• Protein Family: Angiopoietin-related protein
• UniProt Gene Name: ANGPTL3
• UniProt Synonym Gene Names: ANGPT5; ANG-5

NCBI Description

This gene encodes a member of a family of secreted proteins that function in angiogenesis. The encoded protein, which is expressed predominantly in the liver, is further processed into an N-terminal coiled-coil domain-containing chain and a C-terminal fibrinogen chain. The N-terminal chain is important for lipid metabolism, while the C-terminal chain may be involved in angiogenesis. Mutations in this gene cause familial hypobetalipoproteinemia type 2. [provided by RefSeq, Aug 2015]

Uniprot Description

Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism (PubMed:11788823, PubMed:12909640, PubMed:23661675, PubMed:25495645). Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake (). Has a stimulatory effect on plasma triglycerides (TG), which is achieved by suppressing plasma TG clearance via inhibition of LPL activity. The inhibition of LPL activity appears to be an indirect mechanism involving recruitment of proprotein convertases PCSK6 and FURIN to LPL leading to cleavage and dissociation of LPL from the cell surface; the function does not require ANGPTL3 proteolytic cleavage but seems to be mediated by the N-terminal domain, and is not inhibited by GPIHBP1 (PubMed:12097324, PubMed:19318355, PubMed:20581395). Can inhibit endothelial lipase, causing increased plasma levels of high density lipoprotein (HDL) cholesterol and phospholipids (PubMed:17110602, PubMed:19028676). Can bind to adipocytes to activate lipolysis, releasing free fatty acids and glycerol (PubMed:12565906). Suppresses LPL specifically in oxidative tissues which is required to route very low density lipoprotein (VLDL)-TG to white adipose tissue (WAT) for storage in response to food; the function may involve cooperation with circulating, liver-derived ANGPTL8 and ANGPTL4 expression in WAT (). Contributes to lower plasma levels of low density lipoprotein (LDL)-cholesterol by a mechanism that is independent of the canonical pathway implicating APOE and LDLR. May stimulate hypothalamic LPL activity ().