Mouse Amyloid beta A4 precursor protein-binding family A member 3 (APBA3) ELISA Kit | APBA3 elisa kit

Mouse Amyloid beta A4 precursor protein-binding family A member 3 (APBA3) ELISA Kit

Cat. Num. AAA7235330

• Gene Names: APBA3; X11L2; mint3; MGC:15815
• Reactivity: Mouse
• Synonyms: Amyloid beta A4 precursor protein-binding family A member 3 (APBA3); Mouse Amyloid beta A4 precursor protein-binding family A member 3 (APBA3) ELISA Kit; APBA3 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APBA3 elisa kit

Intended Uses: This APBA3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse APBA3. This ELISA kit for research use only!

Principle of the Assay: APBA3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-APBA3 antibody and an APBA3-HRP conjugate. The assay sample and buffer are incubated together with APBA3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APBA3 concentration since APBA3 from samples and APBA3-HRP conjugate compete for the anti-APBA3 antibody binding site. Since the number of sites is limited, as more sites are occupied by APBA3 from the sample, fewer sites are left to bind APBA3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APBA3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for APBA3 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 11342678
• NCBI GeneID: 9546
• NCBI Accession #: NP_004877.1
• NCBI GenBank Nucleotide #: NM_004886.3
• Molecular Weight: 61,454 Da
• NCBI Official Full Name: amyloid beta A4 protein-binding family A member 3
• NCBI Official Synonym Full Names: amyloid beta (A4) precursor protein-binding, family A, member 3
• NCBI Official Symbol: APBA3
• NCBI Official Synonym Symbols: X11L2; mint3; MGC:15815
• NCBI Protein Information: amyloid beta A4 precursor protein-binding family A member 3; mint-3; X11-like 2 protein; adapter protein X11gamma; neuron-specific X11L2 protein; neuronal munc18-1-interacting protein 3; phosphotyrosine-binding/-interacting domain (PTB)-bearing protein; amyloid beta (A4) precursor protein-binding, family A, member 3 (X11-like 2)
• UniProt Protein Name: Amyloid beta A4 precursor protein-binding family A member 3
• Protein Family: Amyloid beta A4 precursor protein-binding family
• UniProt Gene Name: APBA3
• UniProt Synonym Gene Names: MINT3; X11L2; Mint-3
• UniProt Entry Name: APBA3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the X11 protein family. It is an adapter protein that interacts with the Alzheimer's disease amyloid precursor protein. This gene product is believed to be involved in signal transduction processes. This gene is a candidate gene for Alzheimer's disease. [provided by RefSeq, Jul 2008]

Uniprot Description

APBA3: May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-APP. May enhance the activity of HIF1A in macrophages by inhibiting the activity of HIF1AN.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: perinuclear region of cytoplasm

Molecular Function: enzyme inhibitor activity; protein binding; enzyme binding; beta-amyloid binding

Biological Process: synaptic transmission; protein transport; regulation of gene expression; in utero embryonic development; negative regulation of catalytic activity