Typical Testing Data/Standard Curve (for reference only)
Mouse Alkylglycerol monooxygenase (TMEM195) ELISA Kit | TMEM195 elisa kit
Mouse Alkylglycerol monooxygenase (TMEM195) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: TMEM195 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-TMEM195 antibody and an TMEM195-HRP conjugate. The assay sample and buffer are incubated together with TMEM195-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TMEM195 concentration since TMEM195 from samples and TMEM195-HRP conjugate compete for the anti-TMEM195 antibody binding site. Since the number of sites is limited, as more sites are occupied by TMEM195 from the sample, fewer sites are left to bind TMEM195-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TMEM195 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a tetrahydrobiopterin- and iron-dependent enzyme that cleaves the ether bond of alkylglycerols. Sequence comparisons distinguish this protein as forming a third, distinct class of tetrahydrobiopterin-dependent enzymes. Variations in this gene have been associated with decreased glucose-stimulated insulin response, type 2 diabetes, and susceptibility to intracranial aneurysms. [provided by RefSeq, Aug 2012]
Uniprot Description
AGMO: Glyceryl-ether monooxygenase that cleaves the O-alkyl bond of ether lipids. Ether lipids are essential components of brain membranes. Belongs to the sterol desaturase family. TMEM195 subfamily.
Protein type: Oxidoreductase; Endoplasmic reticulum; Membrane protein, multi-pass; Membrane protein, integral; EC 1.14.16.5
Chromosomal Location of Human Ortholog: 7p21.2
Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane
Molecular Function: iron ion binding; glyceryl-ether monooxygenase activity
Biological Process: ether lipid metabolic process; membrane lipid metabolic process; fatty acid biosynthetic process
Research Articles on TMEM195
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Product Notes
The Mouse TMEM195 agmo (Catalog #AAA7244380) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7244380 ELISA Kit recognizes Mouse TMEM195. It is sometimes possible for the material contained within the vial of "Alkylglycerol monooxygenase (TMEM195), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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