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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CD99 antibody.)

Rabbit anti-Human CD99 Polyclonal Antibody | anti-CD99 antibody

CD99 (CD99, HBA71, MIC2, MIC2X, MIC2Y, MSK5X, CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoProtein E2) (APC)

Gene Names
CD99; MIC2; HBA71; MIC2X; MIC2Y; MSK5X
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by affinity chromatography.
Synonyms
CD99; Polyclonal Antibody; CD99 (CD99; HBA71; MIC2; MIC2X; MIC2Y; MSK5X; CD99 antigen; 12E7; E2 antigen; Protein MIC2; T-cell surface glycoProtein E2) (APC); anti-CD99 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human CD99.
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-CD99 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC: 1:50-1:200
WB: 1:500-1:2000
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human CD99.
Conjugate
APC
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using CD99 antibody.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CD99 antibody.)
Product Categories/Family for anti-CD99 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18,848 Da
NCBI Official Full Name
CD99 antigen isoform a
NCBI Official Synonym Full Names
CD99 molecule
NCBI Official Symbol
CD99
NCBI Official Synonym Symbols
MIC2; HBA71; MIC2X; MIC2Y; MSK5X
NCBI Protein Information
CD99 antigen; E2 antigen; surface antigen MIC2; T-cell surface glycoprotein E2; MIC2 (monoclonal antibody 12E7); antigen identified by monoclonal 12E7, Y homolog; antigen identified by monoclonal antibodies 12E7, F21 and O13
UniProt Protein Name
CD99 antigen
Protein Family
UniProt Gene Name
CD99
UniProt Synonym Gene Names
MIC2; MIC2X; MIC2Y
UniProt Entry Name
CD99_HUMAN

NCBI Description

The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2013]

Uniprot Description

CD99: Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. Belongs to the CD99 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.32 and Yp11.3

Cellular Component: focal adhesion; integral to plasma membrane; cytoplasm; plasma membrane

Biological Process: cell adhesion

Research Articles on CD99

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Product Notes

The CD99 cd99 (Catalog #AAA6410536) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD99 (CD99, HBA71, MIC2, MIC2X, MIC2Y, MSK5X, CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoProtein E2) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD99 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). IHC: 1:50-1:200 WB: 1:500-1:2000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CD99 cd99 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD99, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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