Mouse Aldoc (Fructose-bisphosphate aldolase C) ELISA Kit | Aldoc elisa kit

Mouse Aldoc (Fructose-bisphosphate aldolase C) ELISA Kit

Cat. Num. AAA7606765

• Gene Names: ALDOC; ALDC
• Reactivity: Mouse
• Synonyms: Aldoc (Fructose-bisphosphate aldolase C); Mouse Aldoc (Fructose-bisphosphate aldolase C) ELISA Kit; Aldoc elisa kit

• Reactivity: Mouse
• Sequence Length: 364

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Aldoc elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Aldoc antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Aldoc antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Aldoc amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Aldoc can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4885063
• NCBI GeneID: 230
• NCBI Accession #: NP_005156.1
• NCBI GenBank Nucleotide #: NM_005165.2
• UniProt Accession #: P09972; Q3SYL3; Q6FH94; Q6P0L5; B2R5R3
• Molecular Weight: 39,456 Da
• NCBI Official Full Name: fructose-bisphosphate aldolase C
• NCBI Official Synonym Full Names: aldolase, fructose-bisphosphate C
• NCBI Official Symbol: ALDOC
• NCBI Official Synonym Symbols: ALDC
• NCBI Protein Information: fructose-bisphosphate aldolase C
• UniProt Protein Name: Fructose-bisphosphate aldolase C
• Protein Family: Fructose-bisphosphate aldolase
• UniProt Gene Name: ALDOC
• UniProt Synonym Gene Names: ALDC

NCBI Description

This gene encodes a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. [provided by RefSeq, Jul 2008]

Uniprot Description

ALDOC: a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. [provided by RefSeq, Jul 2008]

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; EC 4.1.2.13; Lyase

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cytoskeleton; cytosol; extracellular region

Molecular Function: cytoskeletal protein binding; fructose-bisphosphate aldolase activity; protein binding

Biological Process: epithelial cell differentiation; fructose 1,6-bisphosphate metabolic process; fructose metabolic process; gluconeogenesis; neutrophil degranulation