Typical Testing Data/Standard Curve (for reference only)
Mouse Alanine--glyoxylate aminotransferase 2-like 1 (AGXT2L1) ELISA Kit | AGXT2L1 elisa kit
Mouse Alanine--glyoxylate aminotransferase 2-like 1 (AGXT2L1) ELISA Kit
Gene Names
ETNPPL; AGXT2L1
Reactivity
Mouse
Synonyms
Alanine--glyoxylate aminotransferase 2-like 1 (AGXT2L1); Mouse Alanine--glyoxylate aminotransferase 2-like 1 (AGXT2L1) ELISA Kit; AGXT2L1 elisa kit
Reactivity
Mouse
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AGXT2L1 elisa kit
Intended Uses||This AGXT2L1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse AGXT2L1. This ELISA kit for research use only!
Principle of the Assay: AGXT2L1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AGXT2L1 antibody and an AGXT2L1-HRP conjugate. The assay sample and buffer are incubated together with AGXT2L1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGXT2L1 concentration since AGXT2L1 from samples and AGXT2L1-HRP conjugate compete for the anti-AGXT2L1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AGXT2L1 from the sample, fewer sites are left to bind AGXT2L1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGXT2L1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: AGXT2L1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AGXT2L1 antibody and an AGXT2L1-HRP conjugate. The assay sample and buffer are incubated together with AGXT2L1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGXT2L1 concentration since AGXT2L1 from samples and AGXT2L1-HRP conjugate compete for the anti-AGXT2L1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AGXT2L1 from the sample, fewer sites are left to bind AGXT2L1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGXT2L1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
48,942 Da
NCBI Official Full Name
Alanine-glyoxylate aminotransferase 2-like 1
NCBI Official Synonym Full Names
ethanolamine-phosphate phospho-lyase
NCBI Official Symbol
ETNPPL
NCBI Official Synonym Symbols
AGXT2L1
NCBI Protein Information
ethanolamine-phosphate phospho-lyase; alanine-glyoxylate aminotransferase 2-like 1; alanine--glyoxylate aminotransferase 2-like 1
UniProt Protein Name
Ethanolamine-phosphate phospho-lyase
UniProt Gene Name
ETNPPL
UniProt Synonym Gene Names
AGXT2L1
UniProt Entry Name
AT2L1_HUMAN
Uniprot Description
ETNPPL: Catalyzes the pyridoxal-phosphate-dependent breakdown of phosphoethanolamine, converting it to ammonia, inorganic phosphate and acetaldehyde. Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 4.2.3.2; Transferase
Chromosomal Location of Human Ortholog: 4q25
Cellular Component: mitochondrion
Molecular Function: transaminase activity; ethanolamine-phosphate phospho-lyase activity; pyridoxal phosphate binding
Biological Process: metabolic process
Research Articles on AGXT2L1
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Product Notes
The Mouse AGXT2L1 etnppl (Catalog #AAA7215312) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7215312 ELISA Kit recognizes Mouse AGXT2L1. It is sometimes possible for the material contained within the vial of "Alanine--glyoxylate aminotransferase 2-like 1 (AGXT2L1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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