Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse ADAM DEC1 (ADAMDEC1) Standard Curve.)
Mouse ADAM DEC1 (ADAMDEC1) ELISA Kit | ADAMDEC1 elisa kit
Mouse ADAM DEC1 (ADAMDEC1) ELISA Kit
Gene Names
Adamdec1; Dcsn; AI574231; 2210414L24Rik
Reactivity
Mouse
Synonyms
ADAM DEC1 (ADAMDEC1); Mouse ADAM DEC1 (ADAMDEC1) ELISA Kit; ADAMDEC1; FLJ79219; M12.219; decysin; disintegrin protease; ADAMDEC1 elisa kit
Reactivity
Mouse
Specificity
Mouse ADAM DEC1 (ADAMDEC1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ADAMDEC1. No significant cross-reactivity or interference between Mouse ADAMDEC1 and analogues was observed.
Sequence Length
467
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse ADAM DEC1 (ADAMDEC1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse ADAM DEC1 (ADAMDEC1) Standard Curve.)
Related Product Information for ADAMDEC1 elisa kit
Description: This Mouse ADAM DEC1 (ADAMDEC1) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAMDEC1 in samples. An antibody specific for ADAMDEC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMDEC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMDEC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMDEC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The ADAM family is composed of zinc-binding proteins that can function as adhesion proteins and/or endopeptidases.The prototypic ADAM protein has a prodomain, a metalloprotease domain, a disintegrin domain, a cysteine-rich region, a transmembrane domain, and a variable cytoplasmic tail. ADAMDEC1 belongs to a novel ADAM subfamily due to its partial lack of a disintegrin domain and its total lack of a cysteine-rich domain.ADAMDEC1 protein has 2 unique features compared with other ADAM proteins: the third histidine residue in its zinc-binding site is replaced with an aspartate, and it prematurely terminates in the disintegrin domain, deleting half the disintegrin domain as well as the cysteine-rich domain, the transmembrane domain, and the intracellular tail.
Background: The ADAM family is composed of zinc-binding proteins that can function as adhesion proteins and/or endopeptidases.The prototypic ADAM protein has a prodomain, a metalloprotease domain, a disintegrin domain, a cysteine-rich region, a transmembrane domain, and a variable cytoplasmic tail. ADAMDEC1 belongs to a novel ADAM subfamily due to its partial lack of a disintegrin domain and its total lack of a cysteine-rich domain.ADAMDEC1 protein has 2 unique features compared with other ADAM proteins: the third histidine residue in its zinc-binding site is replaced with an aspartate, and it prematurely terminates in the disintegrin domain, deleting half the disintegrin domain as well as the cysteine-rich domain, the transmembrane domain, and the intracellular tail.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ADAM DEC1
NCBI Official Synonym Full Names
ADAM-like, decysin 1
NCBI Official Symbol
Adamdec1
NCBI Official Synonym Symbols
Dcsn; AI574231; 2210414L24Rik
NCBI Protein Information
ADAM DEC1
UniProt Protein Name
ADAM DEC1
Protein Family
UniProt Gene Name
Adamdec1
UniProt Synonym Gene Names
ADAM-like protein decysin-1
UniProt Entry Name
ADEC1_MOUSE
Uniprot Description
ADAMDEC1: May play an important role in the control of the immune response and during pregnancy. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.24.-; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Protease
Cellular Component: extracellular region
Molecular Function: peptidase activity; zinc ion binding; hydrolase activity; metallopeptidase activity; metalloendopeptidase activity; metal ion binding
Biological Process: proteolysis
Research Articles on ADAMDEC1
Similar Products
Product Notes
The Mouse ADAMDEC1 adamdec1 (Catalog #AAA9717161) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717161 ELISA Kit recognizes Mouse ADAMDEC1. It is sometimes possible for the material contained within the vial of "ADAM DEC1 (ADAMDEC1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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