Human A Disintegrin And Metalloprotease 33 (ADAM33) ELISA Kit | ADAM33 elisa kit

Human A Disintegrin And Metalloprotease 33 (ADAM33) ELISA Kit

Cat. Num. AAA9714580

• Gene Names: ADAM33; C20orf153; DJ964F7.1
• Reactivity: Human
• Synonyms: A Disintegrin And Metalloprotease 33 (ADAM33); Human A Disintegrin And Metalloprotease 33 (ADAM33) ELISA Kit; ADAM33; RP5-964F7.2; C20orf153; DJ964F7.1; DKFZp434K0521; FLJ35308; FLJ36751; MGC149823; MGC71889; a disintegrin and metalloprotease 33; a disintegrin and metalloproteinase domain 33; disintegrin and reprolys; ADAM33 elisa kit

• Reactivity: Human
• Specificity: Human A Disintegrin And Metalloprotease 33 (ADAM33) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADAM33. No significant cross-reactivity or interference between Human ADAM33 and analogues was observed.
• Sequence Length: 813

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human A Disintegrin And Metalloprotease 33 (ADAM33) Standard Curve.)

Related Product Information for ADAM33 elisa kit

Description: This Human A Disintegrin And Metalloprotease 33 (ADAM33) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM33 in samples. An antibody specific for ADAM33 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAM33 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM33 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM33 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: ADAM33 encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. This protein is a type I transmembrane protein implicated in asthma and bronchial hyperresponsiveness. Alternative splicing of this gene results in two transcript variants encoding different isoforms.The 813-amino acid human protein shares 70% identity with the mouse protein. ADAM33 has a domain organization identical to that of previously reported members of the ADAM family, and contains the typical zinc-binding consensus sequence in the metalloprotease domain and a pattern of cysteine localization in the EGF-like domain that is typical of an EGF-like motif. The human protein shows 44% identity with Xenopus ADAM13, 40% with human ADAM19, and 39% with human ADAM12.

NCBI and Uniprot Product Information

• NCBI GI #: 18252045
• NCBI GeneID: 80332
• NCBI Accession #: NP_079496.1
• NCBI GenBank Nucleotide #: NP_079496.1
• UniProt Accession #: Q9BZ11
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 33 isoform alpha preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 33
• NCBI Official Symbol: ADAM33
• NCBI Official Synonym Symbols: C20orf153; DJ964F7.1
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 33
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 33
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM33
• UniProt Synonym Gene Names: C20orf153
• UniProt Entry Name: ADA33_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This protein is a type I transmembrane protein implicated in asthma and bronchial hyperresponsiveness. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2013]