Mouse 3-Phosphoinositide-Dependent Protein Kinase 1 (PDPK1) ELISA Kit | PDPK1 elisa kit
Mouse 3-Phosphoinositide-Dependent Protein Kinase 1 (PDPK1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Background: PDPK1 is a master kinase, which is crucial for the activation of AKT/PKB and many other AGC kinases including PKC, S6K, SGK. An important role for PDPK1 is in the signalling pathways activated by several growth factors and hormones including insulin signalling. Mice lacking PDPK1 die during early embryonic development, indicating that this enzyme is critical for transmitting the growth-promoting signals nescessary for normal mammalian development. Mice that are deficient in PDPK1 have a ?40% decrease in body mass, mild glucose intolerance, and are resitant to cancer brought about by hyperactivation of the PI3K pathway (PTEN +/-).The structure of PDPK1 can be divided into two domains; the kinase or catalytic domain and the PH domain.
NCBI and Uniprot Product Information
Uniprot Description
PDK1: an AGC kinase of the PKB family that contains a PH domain. Involved in a wide variety of processes including cell proliferation, differentiation and apoptosis. Autophosphorylation in the activation loop is necessary for activity. Phosphorylates and activates kinases including Akt, PKC isozymes, p70 S6K and RSK. Three alternatively-spliced isoforms have been described.
Protein type: Nuclear receptor co-regulator; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; AGC group; PKB family
Cellular Component: cell junction; cell projection; cytoplasm; cytoplasmic membrane-bound vesicle; cytosol; membrane; nucleus; perikaryon; plasma membrane
Molecular Function: 3-phosphoinositide-dependent protein kinase activity; ATP binding; insulin receptor binding; kinase activity; nucleotide binding; phospholipase activator activity; phospholipase binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; transferase activity
Biological Process: activation of protein kinase B; calcium-mediated signaling; cell migration; cellular response to insulin stimulus; epidermal growth factor receptor signaling pathway; focal adhesion formation; hyperosmotic response; negative regulation of neuron apoptosis; negative regulation of protein kinase activity; negative regulation of toll-like receptor signaling pathway; negative regulation of transforming growth factor beta receptor signaling pathway; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of release of sequestered calcium ion into cytosol; protein amino acid phosphorylation; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of mast cell degranulation; regulation of transcription, DNA-dependent; signal transduction; transcription, DNA-dependent
Research Articles on PDPK1
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Product Notes
The Mouse PDPK1 pdpk1 (Catalog #AAA9717384) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717384 ELISA Kit recognizes Mouse PDPK1. It is sometimes possible for the material contained within the vial of "3-Phosphoinositide-Dependent Protein Kinase 1 (PDPK1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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