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Western Blot (WB) (Anti-LXN Antibody at 1:1000 dilution + MCF-7 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human LXN Polyclonal Antibody | anti-LXN antibody

LXN Antibody

Gene Names
LXN; ECI; TCI
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
Synonyms
LXN; Polyclonal Antibody; LXN Antibody; Latexin; Endogenous carboxypeptidase inhibitor; ECI; Protein MUM; Tissue carboxypeptidase inhibitor; TCI; anti-LXN antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This LXN antibody is generated from rabbits immunized with human LXN recombinant protein.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
222
Applicable Applications for anti-LXN antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
WB:~~1:1000
IHC-P:~~1:50~100
FC:~~1:10~50
Subtitle
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Cellular Location
Cytoplasm.
Tissue Location
Highly expressed in heart, prostate, ovary, kidney, pancreas, and colon, moderate or low in other tissues including brain.
Antigen Type
Recombinant Protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-LXN Antibody at 1:1000 dilution + MCF-7 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Anti-LXN Antibody at 1:1000 dilution + MCF-7 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)-Paraffin

(LXN Antibody IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LXN Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC)-Paraffin (LXN Antibody IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LXN Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(LXN Antibody flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (LXN Antibody flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-LXN antibody
Description: LXN encodes the only known protein inhibitor of zinc-dependent metallocarboxypeptidases.

Background: Hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4. May play a role in inflammation.
Product Categories/Family for anti-LXN antibody
References
Pallares, I., et al. Proc. Natl. Acad. Sci. U.S.A. 102 (11):3978-3983 (2005). Liu, Q., et al. Mol. Biol. Rep. 27 (4):241-246 (2000).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25750 Da
NCBI Official Full Name
latexin
NCBI Official Synonym Full Names
latexin
NCBI Official Symbol
LXN
NCBI Official Synonym Symbols
ECI; TCI
NCBI Protein Information
latexin
UniProt Protein Name
Latexin
Protein Family
UniProt Gene Name
LXN
UniProt Synonym Gene Names
ECI; TCI
UniProt Entry Name
LXN_HUMAN

NCBI Description

This gene encodes the only known protein inhibitor of zinc-dependent metallocarboxypeptidases. [provided by RefSeq, Oct 2008]

Uniprot Description

LXN: Hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4. Belongs to the protease inhibitor I47 (latexin) family.

Chromosomal Location of Human Ortholog: 3q25.32

Cellular Component: cytoplasm

Molecular Function: heparin binding; metalloendopeptidase inhibitor activity; protein binding

Biological Process: detection of temperature stimulus involved in sensory perception of pain; inflammatory response

Research Articles on LXN

Similar Products

Product Notes

The LXN lxn (Catalog #AAA9217173) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LXN Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LXN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), ELISA (EIA). WB:~~1:1000 IHC-P:~~1:50~100 FC:~~1:10~50. Researchers should empirically determine the suitability of the LXN lxn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LXN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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