Mouse 1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit | DHVD3 elisa kit

Mouse 1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit

Cat. Num. AAA2602146

• Gene Names: CYP24A1; CP24; HCAI; CYP24; P450-CC24
• Reactivity: Mouse
• Synonyms: 1; 25-Dihydroxyvitamin D3 (DHVD3); Mouse 1; 25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit; DHVD3 elisa kit

• Reactivity: Mouse
• Sequence Length: 513

• Samples: Mouse serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10 ng/mL-0.156 ng/mL
• Sensitivity: Up to 0.05 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DHVD3 elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse DHVD3 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 306704
• NCBI GeneID: 1591
• Molecular Weight: 43,051 Da
• NCBI Official Full Name: 1,25-dihydroxyvitamin D3 24-hydroxylase
• NCBI Official Synonym Full Names: cytochrome P450, family 24, subfamily A, polypeptide 1
• NCBI Official Symbol: CYP24A1
• NCBI Official Synonym Symbols: CP24; HCAI; CYP24; P450-CC24
• NCBI Protein Information: 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 24-OHase; cytochrome P450 24A1; cytochrome P450-CC24; vitamin D 24-hydroxylase; exo-mitochondrial protein; vitamin D(3) 24-hydroxylase; 1,25-@dihydroxyvitamin D3 24-hydroxylase; cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase)
• UniProt Protein Name: 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
• UniProt Gene Name: CYP24A1
• UniProt Synonym Gene Names: CYP24; 24-OHase; Vitamin D(3) 24-hydroxylase
• UniProt Entry Name: CP24A_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP24A1: Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25- hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23- hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product. Defects in CYP24A1 are the cause of hypercalcemia infantile (HCAI). HCAI is a disorder characterized by abnormally high level of calcium in the blood, failure to thrive, vomiting, dehydration, and nephrocalcinosis. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.13.126; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 20q13

Cellular Component: mitochondrial inner membrane

Molecular Function: iron ion binding; heme binding; 25-hydroxycholecalciferol-24-hydroxylase activity; oxidoreductase activity; 1-alpha,25-dihydroxyvitamin D3 (1,25-(OH)2D3) 24-hydroxylase activity

Biological Process: osteoblast differentiation; steroid metabolic process; response to vitamin D; vitamin metabolic process; xenobiotic metabolic process; vitamin D metabolic process; vitamin D catabolic process

Disease: Hypercalcemia, Infantile