Western Blot (WB)
(Western Blot: Sample. Lane1: Human Serum; Lane2: Porcine Liver Tissue; Lane3: Human HeLa Cells; Lane4: Porcine Brain Tissue; Lane5: Human MCF-7 Cells.)
Rabbit anti-Human Superoxide Dismutase 2, Mitochondrial (SOD2) Polyclonal Antibody | anti-SOD2 antibody
Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2)
MGHHHHHHSGSEF-KHSLPD LPYDYGALEP HINAQIMQLH HSKHHAAYVN NLNVTEEKYQ EALAKGDVTA QIALQPALKF NGGGHINHSI FWTNLSPNGG GEPKGELLEAIKRDFGSFDK FKEKLTAASV GVQGSGWGWL GFNKERGHLQ IAACPNQDPL QGTTGLIPLL GIDVWEHAYY LQYKNVRPDY LKAIWNVINW ENVTERYMAC KK
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample. Lane1: Human Serum; Lane2: Porcine Liver Tissue; Lane3: Human HeLa Cells; Lane4: Porcine Brain Tissue; Lane5: Human MCF-7 Cells.)
Western Blot (WB)
(Western Blot: Sample. Lane1: Human Serum; Lane2: Porcine Liver Tissue; Lane3: Human HeLa Cells; Lane4: Porcine Brain Tissue; Lane5: Human MCF-7 Cells.)
Western Blot (WB)
(Western Blot: Sample: RecombinantSOD2,Human.)
Western Blot (WB)
(Western Blot: Sample: RecombinantSOD2,Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Glioma Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Glioma Tissue)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type MCF7 cell lysate; Lane 2: SOD2 knockout MCF7 cell lysate; Predicted MW: 18,20,21,25kd Observed MW: 21kd Primary Ab: 3ug/ml Rabbit Anti-Human SOD2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type MCF7 cell lysate; Lane 2: SOD2 knockout MCF7 cell lysate; Predicted MW: 18,20,21,25kd Observed MW: 21kd Primary Ab: 3ug/ml Rabbit Anti-Human SOD2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1. [provided by RefSeq, Apr 2016]
Uniprot Description
SOD2: Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the iron/manganese superoxide dismutase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.15.1.1; Mitochondrial; Oxidoreductase
Chromosomal Location of Human Ortholog: 6q25.3
Cellular Component: extracellular exosome; mitochondrial matrix; mitochondrion
Molecular Function: identical protein binding; manganese ion binding; superoxide dismutase activity
Biological Process: acetylcholine vasodilation involved in regulation of systemic arterial blood pressure; age-dependent response to reactive oxygen species; cellular response to oxidative stress; mitochondrion organization; negative regulation of cell proliferation; negative regulation of neuron apoptosis; negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway; oxygen homeostasis; positive regulation of cell migration; protein homotetramerization; regulation of blood pressure; regulation of transcription from RNA polymerase II promoter; release of cytochrome c from mitochondria; removal of superoxide radicals; response to superoxide; superoxide metabolic process
Disease: Microvascular Complications Of Diabetes, Susceptibility To, 6
Research Articles on SOD2
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Product Notes
The SOD2 sod2 (Catalog #AAA2003307) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Superoxide Dismutase 2, Mitochondrial (SOD2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB), IP, IF, FCM. Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the SOD2 sod2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG SEF-KHSLPD LPYDYGALEP HINAQIMQLH HSKHHAAYVN NLNVTEEKYQ EALAKGDVTA QIALQPALKF NGGGHINHSI FWTNLSPNGG GEPKGELLEA IKRDFGSFDK FKEKLTAASV GVQGSGWGWL GFNKERGHLQ IAACPNQDPL QGTTGLIPLL GIDVWEHAYY LQYKNVRPDY LKAIWNVINW ENVTERYMAC KK. It is sometimes possible for the material contained within the vial of "Superoxide Dismutase 2, Mitochondrial (SOD2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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