Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Sphingomyelin phosphodiesterase 2 (SMPD2) Standard Curve.)
Rat Sphingomyelin Phosphodiesterase 2 (SMPD2) ELISA Kit | SMPD2 elisa kit
Rat Sphingomyelin Phosphodiesterase 2 (SMPD2) ELISA Kit
Gene Names
Smpd2; nSMase
Reactivity
Rat
Synonyms
Sphingomyelin Phosphodiesterase 2 (SMPD2); Rat Sphingomyelin Phosphodiesterase 2 (SMPD2) ELISA Kit; SMPD2; RP5-919F19.2; ISC1; NSMASE; NSMASE1; sphingomyelin phosphodiesterase 2; neutral; SMPD2 elisa kit
Reactivity
Rat
Specificity
Rat Sphingomyelin phosphodiesterase 2 (SMPD2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SMPD2. No significant cross-reactivity or interference between Rat SMPD2 and analogues was observed.
Sequence Length
422
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Sphingomyelin phosphodiesterase 2 (SMPD2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Sphingomyelin phosphodiesterase 2 (SMPD2) Standard Curve.)
Related Product Information for SMPD2 elisa kit
Description: This Rat Sphingomyelin phosphodiesterase 2 (SMPD2) ELISA Kit employs a two-site sandwich ELISA to quantitate SMPD2 in samples. An antibody specific for SMPD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SMPD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMPD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMPD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SMPD2 isa protein which was initially identified as a sphingomyelinase based on sequence similarity between bacterial sphingomyelinases and a yeast protein. Subsequent studies showed that its biological function is less likely to be as a sphingomyelinase and instead as a lysophospholipase. Neutral sphingomyelinase (N-SMase) activity was first described in fibroblasts from patients with Niemann-Pick disease - a lysosomal storage disease characterized by deficiencies in acid SMase. Subsequent study found that this enzyme was the product of a distinct gene, had an optimum pH of 7.4, was dependent on Mg2+ ions for activity, and was particularly enriched in brain.
Background: SMPD2 isa protein which was initially identified as a sphingomyelinase based on sequence similarity between bacterial sphingomyelinases and a yeast protein. Subsequent studies showed that its biological function is less likely to be as a sphingomyelinase and instead as a lysophospholipase. Neutral sphingomyelinase (N-SMase) activity was first described in fibroblasts from patients with Niemann-Pick disease - a lysosomal storage disease characterized by deficiencies in acid SMase. Subsequent study found that this enzyme was the product of a distinct gene, had an optimum pH of 7.4, was dependent on Mg2+ ions for activity, and was particularly enriched in brain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
sphingomyelin phosphodiesterase 2
NCBI Official Synonym Full Names
sphingomyelin phosphodiesterase 2
NCBI Official Symbol
Smpd2
NCBI Official Synonym Symbols
nSMase
NCBI Protein Information
sphingomyelin phosphodiesterase 2
UniProt Protein Name
Sphingomyelin phosphodiesterase 2
UniProt Gene Name
Smpd2
UniProt Synonym Gene Names
Lyso-PAF-PLC; N-SMase; nSMase
UniProt Entry Name
NSMA_RAT
NCBI Description
catalyzes the Mg2+ dependent hydrolysis of sphingomyelin to form N-acylsphingosine and choline phosphate; also has a lower activity for the hydrolysis of lyso-platelet activating factor to 1-alkylglycerol [RGD, Feb 2006]
Uniprot Description
Function: Converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF.
Catalytic activity: Sphingomyelin + H2O = N-acylsphingosine + phosphocholine.
Cofactor: Magnesium.
Pathway: Lipid metabolism; sphingolipid metabolism.
Subcellular location: Membrane; Multi-pass membrane protein.
Sequence similarities: Belongs to the neutral sphingomyelinase family.
Research Articles on SMPD2
Similar Products
Product Notes
The Rat SMPD2 smpd2 (Catalog #AAA9711851) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711851 ELISA Kit recognizes Rat SMPD2. It is sometimes possible for the material contained within the vial of "Sphingomyelin Phosphodiesterase 2 (SMPD2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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