Mouse anti-Duck; Human Episialin Antibody | anti-Muc1 antibody

Mouse anti Episialin

Cat. Num. AAA570464

• Gene Names: Muc1; EMA; CD227; Muc-1
• Reactivity: Duck; Human
• Applications: Flow Cytometry, Functional Assay, Immunoblot, Western Blot, Immunocytochemistry, Immunohistochemistry, Western Blot
• Synonyms: Episialin; Mouse anti Episialin; anti-Muc1 antibody

• Host: Mouse
• Reactivity: Duck; Human
• Isotype: IgG1
• Clone Number: GP1.4
• Specificity: GP1.4 reacts with all glycoforms of Episialin
• Form/Format: Contains 500 ul 0.2 mg/ml purified monoclonal antibody in PBS containing 0.05% BSA and 0,05% sodium azide.
• Sequence Length: 631

• Applicable Applications for anti-Muc1 antibody: Flow Cytometry (FC/FACS), Immunoblotting (IB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Western Blot (WB)
• Application Notes: FC/FACS: 1:10 - 1:50; IHC with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent; IB: 1:25 - 1:250
• Source Note: GP1.4 is a Mouse monoclonal IgG1 antibody derived by fusion of Mouse myeloma cells with spleen cells from a Mouse immunized with Human milk fat globule.
• Conjugation: FITC
• Preparation and Storage: The antibody is shipped at ambient temperature and may be stored at +4 degree C. For prolonged storage prepare appropriate aliquots and store at or below -20 degree C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 degree C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Related Product Information for anti-Muc1 antibody

Episialin is also known as MUC1, MAM-6, CA 15-3, PEM and EMA. It is a transmembrane glycoprotein with a large mucin-like extracellular domain that matures through several intermediate forms geneRated by proteolysis, and sequential addition and processing of numerous O-linked glycans that are heavily sialylated. The gene encoding episialin (MUC1) is highly polymorphic and each allele encodes a product that contains a different number of repeats (between 30 and 90) leading to large differences in molecular weight of the protein. Episialin is present in several secretory epithelial cell types.

Product Categories/Family for anti-Muc1 antibody

Antibody Purification Reagent; Primary Antibodies; Cell Cycle

References

1. Gourevitch, M. M., von Mensdorff-Pouilly, S., Litvinov, S. V., Kenemans, P., van Kamp, G. J., Verstraeten, A. A., and Hilgers, J. (1995). Polymorphic epithelial mucin (MUC-1)-containing circulating immune complexes in carcinoma patients. Br J Cancer 72, 934-9382. Norum, L. F., Varaas, T., Kierulf, B., and Nustad, K. (1998). Carcinoma-associated MUC1 detected by immunoradiometric assays. Tumour Biol 19, 134-146. 3. Norum, L. F., Sauren, A. M., Rye, P. D., and Nustad, K. (2001). New immunoassays for MUC1 in breast cancer, Tumour Biol 22, 216-22.

NCBI and Uniprot Product Information

• NCBI GI #: 199837
• NCBI GeneID: 17829
• Molecular Weight: 64,539 Da
• NCBI Official Full Name: episialin
• NCBI Official Synonym Full Names: mucin 1, transmembrane
• NCBI Official Symbol: Muc1
• NCBI Official Synonym Symbols: EMA; CD227; Muc-1
• NCBI Protein Information: mucin-1
• UniProt Protein Name: Mucin-1
• Protein Family: Mucin
• UniProt Gene Name: Muc1
• UniProt Synonym Gene Names: Muc-1; MUC-1; MUC1-NT; MUC1-alpha; MUC1-beta

Uniprot Description

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, Src and NF-kappaB pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Regulates P53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of P53 and represses P53 activity.CautionO-glycosylation sites are annotated in first sequence repeat only. Residues at similar position are probably glycosylated in all repeats.