Mouse Mannose Binding Lectin (MBL) DIY ELISA Kit | MBL diy elisa kit

Mouse Mannose Binding Lectin (MBL) ELISA Kit DIY Materials

Cat. Num. AAA2089107

• Gene Names: MBL2; MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC
• Reactivity: Mouse
• Synonyms: Mannose Binding Lectin (MBL); Mouse Mannose Binding Lectin (MBL) ELISA Kit DIY Materials; MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2; Soluble(Opsonic Defect); MBL diy elisa kit

• Reactivity: Mouse
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Mannose Binding Lectin (MBL). No significant cross-reactivity or interference between Mannose Binding Lectin (MBL) and analogues was observed.
• Sequence Length: 38

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.067ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for MBL diy elisa kit

Signal transduction; Infection immunity; Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 169335948
• NCBI GeneID: 4153
• Molecular Weight: 26,144 Da
• NCBI Official Full Name: mannose binding lectin, partial
• NCBI Official Synonym Full Names: mannose binding lectin 2
• NCBI Official Symbol: MBL2
• NCBI Official Synonym Symbols: MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC
• NCBI Protein Information: mannose-binding protein C
• UniProt Protein Name: Mannose-binding protein C
• Protein Family: MreB-like protein
• UniProt Gene Name: MBL2
• UniProt Synonym Gene Names: COLEC1; MBL; MBP-C

NCBI Description

This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes mannose and N-acetylglucosamine on many microorganisms, and is capable of activating the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

MBL2: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q21.1

Cellular Component: cell surface; extracellular region; extracellular space

Molecular Function: calcium ion binding; calcium-dependent protein binding; mannose binding; protein binding; receptor binding; serine-type endopeptidase activity

Biological Process: acute-phase response; complement activation; complement activation, lectin pathway; defense response to bacterium; defense response to Gram-positive bacterium; innate immune response; negative regulation of viral reproduction; opsonization

Disease: Mannose-binding Protein Deficiency