Mouse Mannose Binding Lectin (MBL) DIY ELISA Kit | MBL diy elisa kit

Mouse Mannose Binding Lectin (MBL) ELISA Kit DIY Materials

Gene Names

MBL2; MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC

Reactivity

Mouse

Synonyms

Mannose Binding Lectin (MBL); Mouse Mannose Binding Lectin (MBL) ELISA Kit DIY Materials; MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2; Soluble(Opsonic Defect); MBL diy elisa kit

Ordering
Cat. Num. AAA2089107 When autocomplete results are available use up and down arrows to review and enter to select. Touch device users, explore by touch or with swipe gestures. For Research Use Only!

Reactivity

Mouse

Specificity

The Abs in the kit have high sensitivity and excellent specificity for detection of Mannose Binding Lectin (MBL). No significant cross-reactivity or interference between Mannose Binding Lectin (MBL) and analogues was observed.

Sequence Length

Application Notes

Main materials for "Do It Yourself ELISA Kit"

Assay Type

Double-antibody Sandwich ELISA for Antigen Detection

Samples

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Detection Range

0.156-10ng/mL

Sensitivity

0.067ng/mL

Reagent Contents

Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate

Product Usage

1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Other Reagents Required

ELISA/CLIA Support Pack 1. (Catalog MBS2089471)

Preparation and Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for MBL diy elisa kit

Signal transduction; Infection immunity; Immune molecule

NCBI and Uniprot Product Information

NCBI GI #

169335948

NCBI GeneID

4153

Molecular Weight

26,144 Da

NCBI Official Full Name

mannose binding lectin, partial

NCBI Official Synonym Full Names

mannose binding lectin 2

NCBI Official Symbol

MBL2

NCBI Official Synonym Symbols

MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC

NCBI Protein Information

mannose-binding protein C

UniProt Protein Name

Mannose-binding protein C

Protein Family

MreB-like protein

UniProt Gene Name

MBL2

UniProt Synonym Gene Names

COLEC1; MBL; MBP-C

NCBI Description

This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes mannose and N-acetylglucosamine on many microorganisms, and is capable of activating the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

MBL2: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q21.1

Cellular Component: cell surface; extracellular region; extracellular space

Molecular Function: calcium ion binding; calcium-dependent protein binding; mannose binding; protein binding; receptor binding; serine-type endopeptidase activity

Biological Process: acute-phase response; complement activation; complement activation, lectin pathway; defense response to bacterium; defense response to Gram-positive bacterium; innate immune response; negative regulation of viral reproduction; opsonization

Disease: Mannose-binding Protein Deficiency

Research Articles on MBL

1. These results indicate that the mycobacterial antigen 85 complex is a target for ficolins and mannose-binding lectin.

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Product Notes

The Mouse MBL mbl2 (Catalog #AAA2089107) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2089107 DIY ELISA Kit recognizes Mouse MBL. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the MBL mbl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mannose Binding Lectin (MBL), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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