Rabbit anti-Human EIF4A2 Polyclonal Antibody | anti-EIF4A2 antibody

EIF4A2, CT (EIF4A2, DDX2B, EIF4F, Eukaryotic initiation factor 4A-II, ATP-dependent RNA helicase eIF4A-2) (APC)

Cat. Num. AAA6295062

• Gene Names: EIF4A2; DDX2B; EIF4A; EIF4F; BM-010; eIF4A-II; eIF-4A-II
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EIF4A2; Polyclonal Antibody; CT (EIF4A2; DDX2B; EIF4F; Eukaryotic initiation factor 4A-II; ATP-dependent RNA helicase eIF4A-2) (APC); ATP-dependent RNA helicase eIF4A-2; anti-EIF4A2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-EIF4A2 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: EIF4A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 339~369 amino acids from the C-terminal region of human EIF4A2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of EIF4A2 Antibody (C-term) in K562 cell line lysates (35ug/lane). EIF4A2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF4A2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(EIF4A2 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of EIF4A2 (arrow) using rabbit polyclonal EIF4A2 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4A2 gene.)

Related Product Information for anti-EIF4A2 antibody

Eukaryotic initiation factor 4A plays an important role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins. Two highly homologous forms of functional EIF4A genes, Eif4a1 and Eif4a2, have been isolated in mice; yeast cells also possess 2 EIF4A genes, TIF1 and TIF2. The murine Eif4a and yeast TIF genes appear to belong to a DEAD-box gene family, whose members exhibit extensive amino acid similarity and contain the asp-glu-ala-asp (DEAD) sequence. DEAD-box genes have been identified in species ranging from E-coli to humans. Their function appears to be related to transcriptional/translational regulation (referenced from OMIM).

Product Categories/Family for anti-EIF4A2 antibody

Antibodies; Translation Initiation Factors

References

Martins-de-Souza, D., et.al., J Neural Transm 116 (3), 275-289 (2009). Lin, C.J., et.al., Cancer Res. 68 (13), 5326-5334 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 45645183
• NCBI GeneID: 1974
• UniProt Accession #: Q14240; Q53XJ6; Q96B90; Q96EA8; D3DNU9
• Molecular Weight: 407
• NCBI Official Full Name: Eukaryotic initiation factor 4A-II
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4A2
• NCBI Official Symbol: EIF4A2
• NCBI Official Synonym Symbols: DDX2B; EIF4A; EIF4F; BM-010; eIF4A-II; eIF-4A-II
• NCBI Protein Information: eukaryotic initiation factor 4A-II; ATP-dependent RNA helicase eIF4A-2
• UniProt Protein Name: Eukaryotic initiation factor 4A-II
• Protein Family: Eukaryotic initiation factor
• UniProt Gene Name: EIF4A2
• UniProt Synonym Gene Names: DDX2B; EIF4F; eIF-4A-II; eIF4A-II
• UniProt Entry Name: IF4A2_HUMAN

Uniprot Description

EIF4A2: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. Belongs to the DEAD box helicase family. eIF4A subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Translation; Translation initiation; Helicase; EC 3.6.4.13

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: perinuclear region of cytoplasm; eukaryotic translation initiation factor 4F complex; cytosol

Molecular Function: protein binding; translation initiation factor activity; ATPase activity; helicase activity; ATP binding

Biological Process: poly(A) tail shortening; cellular protein metabolic process; translation; viral reproduction; cytokine and chemokine mediated signaling pathway; translational initiation; gene expression; regulation of translational initiation; mRNA catabolic process, deadenylation-dependent decay