Rabbit anti-Human IkappaB-beta Polyclonal Antibody | anti-NFKBIB antibody

IkappaB-beta (Phospho-Thr19) Antibody

Cat. Num. AAA9502433

• Gene Names: NFKBIB; IKBB; TRIP9
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: IkappaB-beta; Polyclonal Antibody; IkappaB-beta (Phospho-Thr19) Antibody; I-kappa-B-beta; IKB-B; IKBB; IkappaBbeta; NF-kappa-BIB; NF-kappaB inhibitor beta; NFKBIB; TR- interacting protein 9; TRIP9; Thyroid receptor interacting protein 9; anti-NFKBIB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: IkappaB-beta (Phospho-Thr19) Antibody detects endogenous levels of IkappaB-beta only when phosphorylated at Thr19.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 338

• Applicable Applications for anti-NFKBIB antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:1000
• Target Modification: Phospho
• Modification Sites: H:T19
• Immunogen: The antiserum was produced against synthesized peptide derived from human IkappaB-beta around the phosphorylation site of Thr19.
• Immunogen Range: Apr-53
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Western Blot (WB)

(Western blot analysis of lysates from 293 cells treated with TNF-a 20ng/ml 30', using IkappaB-beta (Phospho-Thr19) Antibody. The lane on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 340805857
• NCBI GeneID: 4793
• NCBI Accession #: NP_001230045.1
• NCBI GenBank Nucleotide #: NM_001243116.1
• UniProt Accession #: Q15653; Q96BJ7; A8K3F4
• Molecular Weight: 37 kDa
• NCBI Official Full Name: NF-kappa-B inhibitor beta isoform 2
• NCBI Official Synonym Full Names: NFKB inhibitor beta
• NCBI Official Symbol: NFKBIB
• NCBI Official Synonym Symbols: IKBB; TRIP9
• NCBI Protein Information: NF-kappa-B inhibitor beta
• UniProt Protein Name: NF-kappa-B inhibitor beta
• Protein Family: NF-kappa-B inhibitor
• UniProt Gene Name: NFKBIB
• UniProt Synonym Gene Names: IKBB; TRIP9; NF-kappa-BIB; IkB-B; IkB-beta; IkappaBbeta; TR-interacting protein 9; TRIP-9

NCBI Description

The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.