Mouse Apolipoprotein A5 (APOA5) DIY ELISA Kit | APOA5 diy elisa kit

Mouse Apolipoprotein A5 (APOA5) ELISA Kit DIY Materials

Cat. Num. AAA2089225

• Gene Names: APOA5; RAP3; APOAV
• Reactivity: Mouse
• Synonyms: Apolipoprotein A5 (APOA5); Mouse Apolipoprotein A5 (APOA5) ELISA Kit DIY Materials; Apo-A5; APOA-V; APOAV; RAP3; Regeneration-associated protein 3; APOA5 diy elisa kit

• Reactivity: Mouse
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Apolipoprotein A5 (APOA5). No significant cross-reactivity or interference between Apolipoprotein A5 (APOA5) and analogues was observed.
• Sequence Length: 366

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.129ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for APOA5 diy elisa kit

Cardiovascular biology

NCBI and Uniprot Product Information

• NCBI GI #: 262231737
• NCBI GeneID: 116519
• NCBI Accession #: NP_001160070.1
• NCBI GenBank Nucleotide #: NM_001166598.1
• UniProt Accession #: Q6Q788; Q3MIK6; Q6UWK9; Q9UBJ3; B0YIV9
• Molecular Weight: 41,213 Da
• NCBI Official Full Name: apolipoprotein A-V
• NCBI Official Synonym Full Names: apolipoprotein A5
• NCBI Official Symbol: APOA5
• NCBI Official Synonym Symbols: RAP3; APOAV
• NCBI Protein Information: apolipoprotein A-V
• UniProt Protein Name: Apolipoprotein A-V
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA5
• UniProt Synonym Gene Names: RAP3; Apo-AV; ApoA-V

NCBI Description

The protein encoded by this gene is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Oct 2009]

Uniprot Description

APOA5: Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Interacts with GPIHBP1. Up-regulated by PPARA agonists, which are used clinically to lower serum TG (such as fibrates). Liver and plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Lipid-binding; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: chylomicron; extracellular region; extracellular space

Molecular Function: cholesterol binding; cholesterol transporter activity; enzyme activator activity; enzyme binding; heparin binding; lipid binding; low-density lipoprotein receptor binding; phosphatidylcholine binding; phospholipid binding

Biological Process: acylglycerol homeostasis; cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; lipid transport; lipoprotein metabolic process; neurite regeneration; phosphatidylcholine metabolic process; phospholipid efflux; positive regulation of fatty acid biosynthetic process; positive regulation of lipid catabolic process; positive regulation of lipoprotein lipase activity; positive regulation of receptor-mediated endocytosis; regulation of cholesterol absorption; reverse cholesterol transport; tissue regeneration; triacylglycerol catabolic process; triacylglycerol metabolic process

Disease: Hyperlipoproteinemia, Type V; Hypertriglyceridemia, Familial