Mouse PAG1 Antibody | anti-PAG1 antibody

Anti-PAG1 Purified

Cat. Num. AAA1801474

• Gene Names: PAG1; CBP; PAG
• Reactivity: Rat, Cow, Human, Mouse
• Applications: Immunoprecipitation, Western Blot, Immunohistochemistry
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: PAG1; Anti-PAG1 Purified; phosphoprotein membrane anchor with glycosphingoli; CBP; PAG; anti-PAG1 antibody

• Host: Mouse
• Reactivity: Rat, Cow, Human, Mouse
• Isotype: IgG2b
• Clone Number: PAG-C1
• Specificity: The antibody PAG-C1 recognizes an epitope located in the intracellular C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 432

• Applicable Applications for anti-PAG1 antibody: Immunoprecipitation, Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Immunogen: C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
• Preparation and Storage: Store at 2-8 degree C.

Immunohistochemistry (IHC)-Paraffin

(Immunohistochemistry staining (paraffin sections) of PAG/Cbp in germinal center of lymphatic follicle and in dispersed T cells in appendix tissue by monoclonal antibody PAG-C1. Positive signal in T cells.)

Related Product Information for anti-PAG1 antibody

PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.

NCBI and Uniprot Product Information

• NCBI GI #: 63054864
• NCBI GeneID: 55824
• NCBI Accession #: NP_060910.3
• NCBI GenBank Nucleotide #: NP_060910.3
• UniProt Accession #: Q9NWQ8
• NCBI Official Full Name: phosphoprotein associated with glycosphingolipid-enriched microdomains 1
• NCBI Official Synonym Full Names: phosphoprotein membrane anchor with glycosphingolipid microdomains 1
• NCBI Official Symbol: PAG1
• NCBI Official Synonym Symbols: CBP; PAG
• NCBI Protein Information: phosphoprotein associated with glycosphingolipid-enriched microdomains 1
• UniProt Protein Name: Phosphoprotein associated with glycosphingolipid-enriched microdomains 1
• Protein Family: Protein
• UniProt Gene Name: PAG1
• UniProt Synonym Gene Names: CBP; PAG
• UniProt Entry Name: PHAG1_HUMAN

NCBI Description

The protein encoded by this gene is a type III transmembrane adaptor protein that binds to the tyrosine kinase csk protein. It is thought to be involved in the regulation of T cell activation. [provided by RefSeq, Jul 2008]

Uniprot Description

PAG: an ubiquitous type III transmembrane adaptor protein that binds to the tyrosine kinase CSK protein. It is thought to be involved in the regulation of T cell activation.

Protein type: Membrane protein, integral; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 8q21.13

Cellular Component: plasma membrane; integral to membrane; lipid raft

Molecular Function: protein binding; SH3/SH2 adaptor activity; SH2 domain binding

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of signal transduction; signal transduction; negative regulation of T cell activation; T cell receptor signaling pathway; regulation of T cell activation