Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit | TFPI clia kit

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit

Cat. Num. AAA2708756

• Gene Names: Tfpi; EPI; LACI; AW552122; A630013F22Rik
• Reactivity: Mouse
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Tissue Factor Pathway Inhibitor (TFPI); Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit; Tissue Factor Pathway Inhibitor; TFI; EPI; LACI; TFPI1; Lipoprotein-Associated Coagulation Inhibitor; Extrinsic pathway inhibitor; TFPI clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tissue Factor Pathway Inhibitor (TFPI).; No significant cross-reactivity or interference between Tissue Factor Pathway Inhibitor (TFPI) and analogues was observed.
• Sequence Length: 306

• Applicable Applications for TFPI clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Plasma, urine, cell culture supernates and other biological fluids
• Detection Range: 27.4-20,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 12.4pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Factor Pathway Inhibitor (TFPI) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Factor Pathway Inhibitor (TFPI) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Tissue Factor Pathway Inhibitor (TFPI). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tissue Factor Pathway Inhibitor (TFPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tissue Factor Pathway Inhibitor (TFPI) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 33859644
• NCBI GeneID: 21788
• NCBI Accession #: NP_035706.1
• NCBI GenBank Nucleotide #: NP_035706.1
• UniProt Accession #: O54819
• NCBI Official Full Name: tissue factor pathway inhibitor isoform a
• NCBI Official Synonym Full Names: tissue factor pathway inhibitor
• NCBI Official Symbol: Tfpi
• NCBI Official Synonym Symbols: EPI; LACI; AW552122; A630013F22Rik
• NCBI Protein Information: tissue factor pathway inhibitor
• UniProt Protein Name: Tissue factor pathway inhibitor
• Protein Family: Tissue factor pathway inhibitor
• UniProt Gene Name: Tfpi
• UniProt Synonym Gene Names: TFPI; EPI; LACI
• UniProt Entry Name: TFPI1_MOUSE

Uniprot Description

TFPI: Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Secreted; Inhibitor; Secreted, signal peptide

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protease inhibitor activity

Biological Process: hemostasis; negative regulation of peptidase activity; blood coagulation